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HUVEC cell extracts for the investigation of collagen-induced cell signalling

ABSTRACT: We demonstrated that an abundant Collagen type I alpha 1-derived peptide inhibited collagen type I-induced endothelial cell migration in Human Umbilical Vein Endothelial Cells. We then applied mass spectrometry based proteomics to investigate the potential signalling pathways induced by collagen type I (full length), and were inhibited by the Collagen type I alpha 1-derived peptide.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Antonia Vlahou

PROVIDER: MSV000097296 | MassIVE | Wed Mar 12 03:49:00 GMT 2025

SECONDARY ACCESSION(S): PXD061753

REPOSITORIES: MassIVE

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A Naturally Occurring Urinary Collagen Type I Alpha 1-Derived Peptide Inhibits Collagen Type I-Induced Endothelial Cell Migration at Physiological Concentrations.

Devos Hanne H Mina Ioanna K IK Paradeisi Foteini F Makridakis Manousos M Tserga Aggeliki A Mokou Marika M Zoidakis Jerome J Mischak Harald H Vlahou Antonia A Latosinska Agnieszka A Roubelakis Maria G MG

International journal of molecular sciences 20250802 15

Collagen type I (COL(I)) is a key component of the extracellular matrix (ECM) and is involved in cell signaling and migration through cell receptors. Collagen degradation produces bioactive peptides (matrikines), which influence cellular processes. In this study, we investigated the biological effects of nine most abundant, naturally occurring urinary COL(I)-derived peptides on human endothelial cells at physiological concentrations, using cell migration assays, mass spectrometry-based proteomic ...[more]

PMID: 40806611

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