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Growth-rate dependent regulation of anabolism and catabolism in the thermophilic acetogen T. kivui under glucose limitation and phosphate limitation

ABSTRACT: Allocation of resources in the costly and competitive cellular proteome reflects trade-offs in cellular functions. As in many fast-growing bacteria, proteome composition of Escherichia coli growing aerobically on glucose is strictly regulated by growth rate. An increasing anabolic, especially ribosomal, proteome fraction leads to a decreasing catabolic proteome fraction at faster growth, resulting in shifted metabolism. Our systems-level studies of the thermophilic anaerobic acetogen Thermoanaerobacter kivui revealed a substantially different strategy: Over a range of two orders of magnitude, proteome allocation is minimally controlled by growth rate and metabolic rates are primarily controlled posttranslationally. Composition of the catabolic proteome is uncoupled from catabolic rates as indicated by product formation and flux analysis. At slower growth, ribosome numbers are controlled by rRNA concentrations, leading to an excess of ribosomal proteins. This points at a complex landscape of ecophysiological strategies and advantages for a more lenient regulation of proteome allocation.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Thermoanaerobacter Kivui (ncbitaxon:2325)

SUBMITTER: Alfred M. Spormann James R. Williamson

PROVIDER: MSV000097351 | MassIVE | Mon Mar 17 21:57:00 GMT 2025

REPOSITORIES: MassIVE

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