CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation

ABSTRACT: HEK293T cells were co-transfected with StrepII-FLAG-tagged DCAF12 and HA-tagged CUL4A, grown for 48 h, harvested, and lysed as described above. In the first step, Strep-TactinXT Superflow resin (IBA Lifesciences, Gottingen, Germany) was used to purify proteins associated with StrepII-FLAG-tagged subsrtrate receptors. In the second step, eluates from the previous step were used for immunoprecipitation of HA-CUL1/4A-associated proteins. Eluates from both steps were analyzed by LC-MS/MS at the Proteomics facility of Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University (BIOCEV) in Vestec, Czech Republic. Further details are provided in Supplementary methods.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Lukas Cermak  

PROVIDER: MSV000097470 | MassIVE | Tue Apr 01 06:09:00 BST 2025

SECONDARY ACCESSION(S): PXD067954

REPOSITORIES: MassIVE