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CRL4-DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication

ABSTRACT: HEK293T cells were transfected with StrepII-FLAG-tagged DCAF12 and, where indicated, treated with the proteasome inhibitor MG-132 for 6 hours. Following treatment, cells were harvested and lysed. Protein complexes associated with StrepII-FLAG-tagged DCAF12 were purified using Strep-TactinXT Superflow resin (IBA Lifesciences, Gottingen, Germany) and eluted with desthiobiotin. Eluted samples were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis at the Proteomics Facility of the Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University (BIOCEV) in Vestec, Czech Republic.

INSTRUMENT(S): Orbitrap Ascend

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Lukas Cermak

PROVIDER: MSV000097469 | MassIVE | Tue Apr 01 05:26:00 BST 2025

SECONDARY ACCESSION(S): PXD055947

REPOSITORIES: MassIVE

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