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Differential gene expression in Clostridium autoethanogenum due to sugar co-metabolism

ABSTRACT: This experiment includes RNA-seq and metabolomics from a pure microbial strain to examine differential gene expression as a result of pre-adaptation and exposure to multiple carbon sources. This data will provide molecular insight into gene regulation and potential metabolic activity of Clostridium autoethanogenum, an important microbe for the bioenergy industry. C. autoethanogenum plays a unique role in lignocellulosic-based bioproduct synthesis due to its ability to consume single carbon gases as well as pentose and hexose sugars. Our preliminary research has demonstrated the organism’s ability to co-metabolize the pentose and hexose sugars xylose and fructose. However, different responses were observed depending on pre-adaptation or culturing history - whether cultures were grown on either xylose or fructose prior to the dual-sugar experiment. Sugar consumption as well as ethanol and acetate production differed between cultures pre-adapted on xylose versus fructose prior to the xylose-fructose fermentation. Fructose pre-adaptation resulted in both a faster sugar consumption and greater total consumption of sugar. Fructose pre-adapted cultures also produced three times more ethanol and two times more acetate. The work (proposal:https://doi.org/10.46936/10.25585/60000475) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Clostridium Autoethanogenum

SUBMITTER: Ashley Beck

PROVIDER: MSV000097717 | MassIVE | Thu Apr 24 13:48:00 BST 2025

REPOSITORIES: MassIVE

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Differential gene expression in Clostridium autoethanogenum due to sugar co-metabolism

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