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Overexpression of the signaling coordinator GAB2: a missing link in AML progression

ABSTRACT: TurboID experiments in pre-leukemic bone marrow cells of various genotypes, including wildtype, Dnmt3aR878H/+ and DnmtaR878H/+ x Npm1cA/+. We performed proximity labeling experiments using the TurboID system to label intracellular proteins that are in close proximity to GAB2, NPM1wt, NPM1cA, DNMT3Awt or DNMT3AR882H protein (baits), in primary murine HSPCs. We used a TurboID cDNA fused in frame to the N- or C-terminus of each bait in an MSCV-based retroviral vector, and transduced these vectors into primary murine bone marrow HSPCs from relevant genotypes. TurboID cDNA alone was used as a control for non-specific biotin labeling. Cells were lysed and labeled proteins were captured using streptavidin beads, followed by on-bead digest and mass spectrometry.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Timothy J. Ley

PROVIDER: MSV000097966 | MassIVE | Wed May 21 18:31:00 BST 2025

SECONDARY ACCESSION(S): PXD064174

REPOSITORIES: MassIVE

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