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Paraisariamides: molecular cartography of a wild-harvested insect-fungus specimen (beetle larva-P. Insignis)

ABSTRACT: A molecular cartography workflow was used to visualize the spatial distribution of paraisariamides in a lyophilized specimen of a beetle larva parasitized by P. insignis. These heptapeptides were localized to the beetle larva host and fungal endosclerotium inside the larva, consistent with a role in disrupting eukaryotic protein synthesis. LCMS2 data were acquired for 120 samples. Excisions of approximately 1-5 mg of material were made from points on the insect-fungus specimen corresponding to each of the sample points and annotated for the tissue of origin either as fertile portion of the fruiting body (FP), sterile portion of the fruiting body (St), rhizomorphs (Rz), larva exoskeleton (Ex), larva cuticle (Cu), smooth hyphae (Sm), endosclerotium (En), or a combination of two of these tissues.

INSTRUMENT(S): 6545 Q-TOF LC/MS

ORGANISM(S): Paraisaria Insignis

SUBMITTER: Kerry McPhail

PROVIDER: MSV000098205 | MassIVE | Sun Jun 15 20:48:00 BST 2025

REPOSITORIES: MassIVE

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Paraisariamides: molecular cartography of a wild-harvested insect-fungus specimen (beetle larva-P. Insignis)

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