Deep and Quantitative Proteomic Profiling of Low Volume Mouse Serum Across the Lifespan

ABSTRACT: We developed a nanoparticle-based LC-MS workflow optimized for low-volume mouse serum samples, enabling deep, quantitative, and reproducible proteome profiling. Applied to aging cohorts, the approach identified 3,992 protein groups across all samples and uncovered age-associated proteomic signatures in serum and linked to glucose and body composition. Complementary analysis using a 48-plex proximity extension assay (PEA) quantified an additional 39 cytokines not detected by MS, enhancing proteome coverage. This integrated platform supports robust biomarker discovery and systems-level insights in volume-limited preclinical studies.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Dr. Nathan Basisty  

PROVIDER: MSV000098399 | MassIVE | Wed Jul 02 01:05:00 BST 2025

REPOSITORIES: MassIVE