Proteomics

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Mass Spectrometry-Based Mapping of Conformational Epitopes on SARS-CoV-2 Antigens Targeted by Monoclonal Antibodies


ABSTRACT: This study utilized diethylpyrocarbonate covalent labeling mass spectrometry (DEPC CL-MS) to map epitopes on the SARS-CoV-2 spike glycoprotein, focusing on the receptor-binding domain (RBD) of beta (B.1.351) and omicron (B.1.1.529) variants, as well as the original-variant S1 subunit. DEPC labeling with LC-MS/MS identified antibody-binding sites and conformational shifts, revealing significant RBD flexibility and more consistent S1 labeling.

INSTRUMENT(S): QExactive LCMS

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Patanachai Limpikirati  

PROVIDER: MSV000098567 | MassIVE | Sun Jul 20 20:08:00 BST 2025

REPOSITORIES: MassIVE

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