Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

S. elongatus PCC 7942 Limited Proteolysis Structural Proteomics (JM-PB-DP3), TPP-MS-TMT10

ABSTRACT: The purpose of this experiment was to investigate structural alterations in proteins involved in central carbon metabolism and photosynthetic electron transfer pathways in Synechococcus elongatus PCC 7942. Sample data was obtained from S. elongatus PCC 7942 cell lysates treated across 10 temperatures (37 to 82 deg C in 5 deg C increments, prepared using thermal proteome profiling by mass spectrometry (TPP-MS), and fractionated for TMT10 proteomic analysis. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline, followed by Tandem Mass Tag 10-Plex (TMT10) quantitation using PlexedPiper.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Synechococcus Elongatus Pcc 7942 (ncbitaxon:1140)

SUBMITTER: John T. Melchior Snigdha Sarkar

PROVIDER: MSV000098679 | MassIVE | Tue Jul 29 20:02:00 BST 2025

SECONDARY ACCESSION(S): PXD066739

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression of OsTPX Gene Improves Cellular Redox Homeostasis and Photosynthesis Efficiency in Synechococcus elongatus PCC 7942

Project description:The goals of this study are to compare hydrogen peroxide (H2O2) stress tolerance through gene expression of wild type (WT) and OsTPX-expressing Synechoccous elongatus PCC 7942 (OT) under the normal and stress condition.

2018-12-26 | GSE122841 | GEO

Transcriptome analysis of Synechococcus elongatus PCC 7942 for nitrogen starvation response using RNA sequencing

Project description:We reported transcriptome analysis of S. elongatus PCC 7942 for nitrogen starvation response by performing RNA-sequencing. As a result, we identified differentially express genes and confirmed physiological response changes for up or down regulation genes. among differentially expressed genes (p value < 0.01) during N starvation

2017-02-14 | GSE79726 | GEO

Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942

Project description:An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the ascorbate-glutathione cycle, regulate redox status in cells to guard against such damage. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzes the glutathione-dependent reduction of oxidized ascorbate (dehydroascorbate) and contains a redox active site and glutathione binding-site. The DHAR gene is important in biological and abiotic stress responses involving reduction of the oxidative damage caused by ROS. In this study, a transgenic microalgae (TA) strain was constructed by cloning the Oryza sativa L. japonica DHAR (OsDHAR) gene controlled by an isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter (Ptrc) into the Synechococcus elongatus PCC 7942 strain of cyanobacteria to study the functional activities of OsDHAR under oxidative stress caused by hydrogen peroxide exposure. OsDHAR expression increased the growth of S. elongatus PCC 7942 under oxidative stress by reducing the levels of hydroperoxides and malondialdehyde (MDA) and mitigating the loss of chlorophyll. DHAR and glutathione S-transferase activity were higher than in the wild-type (WT) strain. Additionally, overexpression of OsDHAR in S. elongatus PCC 7942 greatly increased the glutathione (GSH)/glutathione disulfide (GSSG) ratio compared to ascorbic acid (AsA)/dehydroascorbate (DHA) ratio in the presence or absence of hydrogen peroxide. These results strongly suggest that DHAR attenuates deleterious oxidative effects via the glutathione (GSH)-dependent antioxidant system in cyanobacterial cells. The expression of heterologous OsDHAR in S. elongatus PCC 7942 protected cells from oxidative damage through a GSH-dependent antioxidant system via GSH-dependent reactions at the redox active site and GSH binding site residues during oxidative stress.

2019-11-25 | GSE140121 | GEO

S. elongatus PCC 7942 Circadian Thermo Proteo Profiling (TPP) Proteomics - Light vs Dark

Project description:Microfractionated TMT10-labeled samples from TPP experimental assays investigating structural alterations in Light vs Dark growth conditions. Samples are Synechococcus elongatus PCC 7942 cell lysates (BTO:0004304). Purpose: To evaluate structural proteome responses under light and dark conditions. Technique: Thermal Proteome Profiling (TPP). Other details: Labeled with TMT-10 kit, Microfractionated into 6 fractions for each plex, There were 6 plexes (3 light, 3 dark) in total. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

2025-11-14 | MSV000099906 | MassIVE

Gene Expression Profiling of Synechococcus elongatus PCC 7942 and Rhodotorula toruloides NBRC 0880 (Rhodosporidium toruloides IFO0880) in Co-culture and Membrane-Separated Bioreactor Configurations Under Various Stress and Nutrient Conditions

Project description:This RNA-seq study examines transcriptional responses of a cyanobacterium–yeast co-culture system comprising Synechococcus elongatus PCC 7942 and Rhodotorula toruloides NBRC 0880 (Rhodosporidium toruloides IFO0880) under multiple conditions. Experiments included planktonic co-cultures in 50 mL tubes subjected to chemical stressors (oxidative stress, photosynthesis inhibition), temperature stress, nutrient limitation (nitrogen, phosphorus), pH extremes, and heterotrophic conditions, as well as physically separated co-cultures in a 600 mL membrane bioreactor. Axenic controls for Synechococcus elongatus PCC 7942 were included for comparison. Differential gene expression analysis provides insight into interspecies interactions and individual organism responses across co-culture configurations.

2026-06-11 | GSE335065 | GEO

Synechococcus elongatus PCC 7942 = FACHB-805

Project description:Synechococcus elongatus PCC 7942 Raw sequence reads

| PRJNA1240180 | ENA

Improved salt tolerance of Synechococcus elongatus PCC 7942 by heterologous synthesis of compatible solute ectoine

Project description:In order to explore the effect of introducing the ectoine synthesis module in Synechococcus elongatus PCC 7942 on the global regulation of the strain under high-salt environment. The ectoine synthesis module was introduced at the Synpcc7942_0808 locus to obtain the Syn7942/Δsps-ect strain that lacked sucrose synthesis and could synthesize ectoine. Comparative transcriptomic analysis was performed on WT and Syn7942/Δsps-ect under 0 mM and 300 mM NaCl conditions.

2023-01-08 | GSE222067 | GEO

Synechococcus elongatus PCC 7942 = FACHB-805

Project description:Synechococcus elongatus PCC 7942 = FACHB-805 Genome sequencing

| PRJNA268360 | ENA

Transcriptomic and phenomic investigations reveal elements in biofilm repression and formation in the cyanobacterium Synechococcus elongatus PCC 7942 [RNA-seq]

Project description:Biofilm formation by photosynthetic organisms is a complex behavior that serves multiple functions in the environment. Biofilm formation in the unicellular cyanobacterium Synechococcus elongatus PCC 7942 is regulated in part by a set of small secreted proteins that promotes biofilm formation and a self-suppression mechanism that prevents their expression. Little is known about the regulatory and structural components of the biofilms in PCC 7942, or response to the suppressor signal(s). We performed transcriptomics (RNA-Seq) and phenomics (RB-TnSeq) screens that identified four genes involved in biofilm formation and regulation, more than 25 additional candidates that may impact biofilm formation, and revealed the transcriptomic adaptation to the biofilm state. In so doing, we compared the effectiveness of these two approaches for gene discovery.

2022-06-06 | GSE205444 | GEO