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Binding proteins of Hydroxymethylcytosine-modified CpG dyads from mouse brain tissue

ABSTRACT: Cytosine (C) modifications within CpG dyads are central regulatory elements of mammalian genomes. In addition to 5-methylcytosine (mC), genomes can also contain high levels of 5-hydroxymethylcytosine (hmC) that is read differently by nuclear proteins than mC. However, hmC can co-exist with C and mC in different combinations in the two strands of CpG dyads, such as hmC/C, hmC/mC, or hmC/hmC, and it is poorly understood, how these combinatorial marks differ in their protein interactomes and regulatory functions. To systematically identify nuclear proteins that bind to distinct hmC-containing CpG dyads, we conducted DNA pulldown experiments using promoter probes harboring defined dyad modifications. In this study, we used nuclear extracts from mouse brain tissue and a DNA probe based on part of the Sp1 promoter sequence. For each dyad configuration, C/C, mC/mC, hmC/mC, hmC/C, and hmC/hmC, five technical replicates were performed.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Petra Janning

PROVIDER: MSV000098682 | MassIVE | Wed Jul 30 07:16:00 BST 2025

SECONDARY ACCESSION(S): PXD066764

REPOSITORIES: MassIVE

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Binding proteins of Hydroxymethylcytosine-modified CpG dyads

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