Proteomics

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Specialized metabolome data of dry seeds from WT, aop3, scpl17 and bzo1 genotypes developed under high temperatures


ABSTRACT: Arabidopsis thaliana (thale cress) wild-type (WT) and transgenic lines were in the Columbia-0 (Col-0) background. The T-DNA insertion mutants of AOP3 (SALK_001655C), BZO1 (two independent lines, SALK_94196 and GABI_56B09) and SCPL17 (two lines independent, SALK_75481 and SALK_126264) and the WT were sowed directly in fertilized soil. Plants were grown in optimal conditions (control) in a growth chamber with long day photoperiod (16h light/21 celsius degrees - 8h dark/19 celsius degrees). Watering was made with water. At the onset of flowering (occurring between 5 and 6 weeks after seed sowing), high temperature (HT) was applied to half of the plants of the corresponding experiment by transferring them to another growth chamber (SANYO, Thermo Fisher Scientific) displaying high temperature conditions (16h of light/27 celsius degrees - 8h of dark/24 celsius degrees). The other half of the plants, constituting control plants, remained in the initial growth chamber with control temperature conditions. Dry seeds were harvested. Untargeted metabolomics (LC-MS/MS) were performed with those samples of dry seeds.

INSTRUMENT(S): impact II, Ultimate 3000 (Thermo Fischer Scientific - HPLC)

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Massimiliano CORSO  

PROVIDER: MSV000099408 | MassIVE | Tue Oct 07 08:42:00 BST 2025

REPOSITORIES: MassIVE

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