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Global profiling of post-translationally modified crustacean neuropeptidome trends affiliated with feeding activity

ABSTRACT: Neuropeptides modulate a diverse range of physiological functions, such as those associated with feeding. Post-translational modifications (PTMs) are responsible for much of the dynamic nature of neuropeptide isoforms for varied influence on these functions. Mass spectrometry is the gold standard analytical technique for peptidomic analyses, followed by computational methods for peptide identification. However, the computational search space quickly becomes difficult to manage as more modifications are considered. With innovative approaches to endless combinations of potential modifications, such as the PEAKS PTM, we globally profiled the Cancer borealis (Jonah crab) neuropeptidome to investigate the role of PTMs in feeding and appetite related processes across time. Through deep examination of several notable modifications, we were able to propose PTM-associated motifs for neuropeptides, which can bolster future identification abilities. Furthermore, this work revealed neuropeptides which were characteristically modified depending on the crab feeding status and the time post-feeding, pointing to potential biological significance. This is the first large-scale investigation of the modified crustacean neuropeptidome, revealing new insights on the implications of modifications in biological regulation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Cancer Borealis (ncbitaxon:39395)

SUBMITTER: Lingjun Li

PROVIDER: MSV000099803 | MassIVE | Sun Nov 09 13:20:00 GMT 2025

SECONDARY ACCESSION(S): PXD070503

REPOSITORIES: MassIVE

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