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On the utility of infrared photoactivation for native top-down and complex-down Orbitrap mass spectrometry of soluble proteoform complexes

ABSTRACT: We implemented infrared (IR) activation for the analysis of soluble multi proteoform complexes (MPCs): alcohol dehydrogenase (ADH; 147 kDa tetramer), enolase (96 kDa dimer), and pyruvate kinase (PK; 232 kDa tetramer). IR photons were used to induce infrared multiphoton dissociation (IRMPD) and to enhance electron-based fragmentation via activated-ion ETD (AI-ETD), and performance was benchmarked against higher-energy collisional dissociation (HCD).

INSTRUMENT(S): Orbitrap Ascend

ORGANISM(S): Oryctolagus Cuniculus (ncbitaxon:9986) Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Luca Fornelli

PROVIDER: MSV000099804 | MassIVE | Sun Nov 09 13:43:00 GMT 2025

REPOSITORIES: MassIVE

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