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POP5 dysregulation causes syndromic immunodeficiency associated with defects in ribosomal and transfer RNA processing and telomere length

ABSTRACT: The goal of this study was to investigate the molecular role of human POP5 in immune cell function, with a particular focus on identifying interaction partners and disease associated mutations that could alter POP5 activity. We performed comprehensive interactomics analyses on both wild type POP5 and selected disease associated POP5 mutants. POP5 constructs were generated with N and C terminal MAC tags to enable affinity purification and proximity labeling. Stable HEK293 cell lines expressing the POP5 variants were established using the MAC tag system, followed by doxycycline inducible expression where applicable. For interactome mapping, we employed affinity purification–mass spectrometry (AP MS) and BioID based proximity labeling to capture both stable and transient interaction partners. Purified protein complexes were analyzed using high resolution LC MS/MS, and interaction networks were constructed using SAINT, CRAPome filtering, and STRING based functional clustering. Differential interactome analysis was performed to compare wild type and mutant POP5, identifying mutation specific interaction changes.

INSTRUMENT(S): timsTOF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Markku Varjosalo

PROVIDER: MSV000100087 | MassIVE | Wed Dec 03 09:49:00 GMT 2025

REPOSITORIES: MassIVE

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