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Ischemic cardiac organoid proteome map

ABSTRACT: In this study, we demonstrate the use of human cardiac organoids (comprised of cardiomyocyte, fibroblast, and endothelial origin) to model IR injury through a model of hypoxia and reoxygenation and therapeutic nanovesicle remodelling. Engineered nanovesicles (NVs), generated directly from human stem cells (SC), have been shown to influence cardiac tissue repair, and provide a platform for the reproducible, rapid, and scalable cell free-mediated therapy. Functionally, we demonstrate that administration of NVs (from different human induced pluripotent stem cell (iPSC) origin) during reoxygenation significantly increase cardiomyocyte survival and preserve contractility function (contractile duration, relaxation time, relaxation:contraction velocity). A mass-spectrometry-based proteomics approach was applied to decipher protein dynamics and molecular mechanisms of IR injury in human cardiac organoids following NV treatments

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: David Greening

PROVIDER: MSV000100182 | MassIVE | Wed Dec 10 20:39:00 GMT 2025

SECONDARY ACCESSION(S): PXD071853

REPOSITORIES: MassIVE

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