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Soil microbiome pH perturbation proteomics

ABSTRACT: Examined soil microbiome microcosms to determine the effect of changing pH on the production of lignocellulolytic enzymes. Soil from a Prosser, Washington field site was incubated in mesh bags on top of a soil interfacing glass bead matrix with MOPS minimal media amended with or without carboxymethyl cellulose at three different pH levels. After incubation, 2 mL of media solution was collected for proteomics, separating intracellular proteome from extracellular proteome via centrifugation. Data was searched with MS-GF+ and MASIC using PNNL's DMS Processing pipeline. Quantitative values were determined using MS1 abundance profiles created by MASIC for every MS2 spectrum, allowing for area under the curve assessments of peptide elution.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Environmental Samples <bacteria> (ncbitaxon:48479)

SUBMITTER: Ryan S. McClure Nicholas J. Reichart

PROVIDER: MSV000100832 | MassIVE | Fri Feb 13 01:06:00 GMT 2026

SECONDARY ACCESSION(S): PXD074420

REPOSITORIES: MassIVE

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