The lipid transfer protein STARD7 controls intestinal tumor development in a contextdependent manner

ABSTRACT: lipid extracts were then individually transferred to new vials and separated on a Dionex UltiMate 3000 LC System (Thermo Scientific, Waltham, Massachusetts, EUA) using a Kinetex C18 EVO 2.6uM, 100A, 150 x 0.3 mm LC Column. Mobile phases consist of (A) H2O - 5mM ammonium formate, 0.1% Formic acid, (B) 60% Acetonitrile: 40% Methanol - 5mM ammonium formate, 0.1% Formic acid, and (C) Isopropanol - 5mM ammonium formate, 0.1% Formic acid. Detailed information on the gradient (time and Flow) are available upon request. Mass spectrometry data were acquired on a Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific, Waltham, Massachusetts, EUA) using data dependent acquisition in positive and negative ion mode. The parameters used were: Full MS: Resolution: 70,000; AGC target: 1e6; Max IT: 100 ms; Scan range: 90 to 1350 m/z. dd-MS2: Resolution: 17,500; AGC target: 1e5; Max IT: 120 ms; Loop count: 5; TopN: 5; Isolation window: 1.5 m/z. Data were processed using compound discovery (Thermo Scientific, Waltham, Massachusetts, EUA) and lipids identification was performed using Lipidex

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Alain Chariot  

PROVIDER: MSV000100938 | MassIVE | Mon Feb 23 06:51:00 GMT 2026

REPOSITORIES: MassIVE