MTBLS11445: Integrative omics reveals distinct metabolic and microbial signatures in broilers under gut health interventions

ABSTRACT: Maintaining a well-balanced gut microbiota is crucial for ensuring optimal health and performance in poultry production. Common strategies supporting gut health include control methods for coccidiosis which is a common and economically important enteric disease in poultry. These interventions not only affect the gut microbial community but are also expected to influence metabolite levels, reflecting the dynamic relationship between gut microorganisms and metabolic processes. In this study, we compared the caecal content metabolite levels between birds given ionophores (T1) and vaccination against Eimeria species (T2), using liquid chromatography-mass spectrometry based untargeted metabolomic analysis. All statistical methods were on performed on R v4.4.1. Data was normalised using the probabilistic quotient method and subjected through Sparse Partial Least Squares Discriminant Analysis (sPLS-DA) and Data Integration Analysis for Biomarker discovery using Latent Components (DIABLO) analyses using the mixomics package. Pathway analysis was also performed using MetaboAnalystR package. Our analysis revealed that metabolites classified as prenol lipids as robust markers for T1, while indole derivatives were identified for T2. KEGG pathways involving amino acids (Tryptophan, Phenylalanine) were also enriched in both groups. We identified Bacillus fragilis to have the greatest impact on the metabolome, positively correlated to >240 metabolites, while UBA3818 spp. (Ruminococcaceae) was shown to have a significant negative relationship with 22 metabolites. Overall, the results indicated that differences in coccidiosis control elicit diverse metabolic events in broilers through its influences on nutrient breakdown, affecting the performance and health of broilers and may provide novel avenues for gut health interventions against Eimeria.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - positive - reverse phase, Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS11445 | MetaboLights | 2025-05-23

REPOSITORIES: MetaboLights