WLJP-025p Promote Colonic Epithelial Repair in Ulcerative Colitis through Fatty Acid Oxidation by Regulating Microbial-involvemented SPD Production

ABSTRACT:

BACKGROUND & AIMS: Ulcerative colitis (UC) poses a serious threat to human health. WLJP-025p, a polysaccharide component of Lonicera japonica thunb. has been confirmed to have anti-inflammatory and immunomodulatory effects. This study intends to explore the effects and mechanism of WLJP-025p in postponing UC progression by the gut microbiota.

METHODS: The UC model was established by dextran sulfate sodium (DSS) treatment. The fecal microorganisms were detected respectively by adopting the 2bRAD-M technology, and the metabolites were detected by using the H650 medical high-throughput targeted detection. The differentially expressed metabolites (spermidine, SPD) screened out are quantitatively detected by LC-MS. The intestinal microorganisms were cultured in vitro under hypoxic conditions to detect the temporal and spatial changes of WLJP-025p decomposition and spermidine generation. Serum biochemistry and flow cytometry are used to detect inflammatory factors. Immunofluorescence and Western blot are used to assess the intestinal mechanical barrier and the molecular mechanism of WLJP-025p.

RESULTS: WLJP-025p dose-dependently mitigate the symptoms of UC mice, restored the balance of the intestinal microbial population and metabolic disorder in UC. WLJP-025p restored the SPD level and the involvement of microorganisms was sufficient and necessary. SPD activated FAO promoted maturation differentiation of intestinal epithelium, and facilitates barrier repair.

CONCLUSIONS: WLJP-025p restores the SPD balance involved by microorganisms, drives FAO and repairs lipid metabolism disorders and damages. It effectively postpone the progression of UC through the mechanisms of gut microbiota composition and metabolic remodeling.

INSTRUMENT(S): Liquid Chromatography MS - alternating - hilic, Liquid Chromatography MS - alternating - reverse phase

PROVIDER: MTBLS12400 | MetaboLights | 2026-05-16

REPOSITORIES: MetaboLights