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Combined analysis of transcriptome and metabolome reveals the accumulation of related metabolites and gene regulation networks during faba bean seed development

ABSTRACT:

Background: Faba bean seeds are nutrient-dense, serving as a vital plant-based protein source for food and vegetables. However, the metabolic accumulation and transcriptional regulatory networks during seed development remain poorly understood.

Results: Metabolomic analysis of two developmental stages in the vegetable faba bean TongCanXian No.7 (TCX) and the grain faba bean QiDou No.2 (QD) identified 868 metabolites. Key metabolic pathways such as flavonoid biosynthesis, flavone and flavonol biosynthesis, ABC transporters, and amino acid metabolism were highlighted. Transcriptomic analysis revealed 37,457 genes with 9,862 differentially expressed genes (3,550 up-regulated and 4,655 down-regulated between the 45 days after anthesis (DAA) and 30 DAA phases). These genes were notably enriched in pathways including starch and sucrose metabolism, flavonoid biosynthesis, and glutathione metabolism. Specifically, within the flavonoid biosynthesis pathway, 19 differentially accumulated metabolites and 34 differentially expressed genes were identified.

Conclusion: This study delivers a robust theoretical foundation for elucidating the molecular and metabolic development in faba bean seeds, thereby advancing our understanding of seed biology and its potential applications in improving nutritional quality.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12471 | MetaboLights | 2025-09-02

REPOSITORIES: MetaboLights

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			Action	DRS
	NEG_Ef15ZN_QC01.raw	Raw
	NEG_Ef15ZN_QC02.raw	Raw
	NEG_Ef15ZN_QC03.raw	Raw
	NEG_Ef15ZN_QC04.raw	Raw
	NEG_Ef15ZN_QD30d1.raw	Raw

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Combined analysis of transcriptome and metabolome reveals the accumulation of related metabolites and gene regulation networks during faba bean seed development

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