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Small auxin-up RNA promotes GmMYB176 gene transcription to modulate soybean isoflavone content

ABSTRACT:

Isoflavones have been widely studied because of their potential health benefits, including antioxidant, anti-inflammatory, and anticancer properties. Soybeans (Glycine max (L.) Merr) are an effective source of natural isoflavones in humans. Therefore, it is important to cultivate varieties showing increased isoflavone contents and elucidate the secondary metabolic pathways of soybean isoflavones. The small auxin-up RNA (SAUR) gene family is the largest family in the auxin response. However, few reports have described their roles in soybean flavonoid metabolism. In this study, the contents of 200 flavonoid target metabolites in GmSAUR:GmSAUR transgenic soybeans were determined. The results showed that the transgenic soybeans had higher daidzein and genistin contents. Chromatin immunoprecipitation sequencing identified potential targets, including GmMYB176. GmSAUR bound to the promoter of GmMYB176; activated the expression of cinnamate 4-hydroxylase, isoflavone-7-O-beta-glucoside 6′′-O-malonyltransferase, cytochrome P450 84A1-like, and cytochrome P450 84A1; and promoted the accumulation of soybean isoflavones. These results suggested that the GmSAUR gene promoted the accumulation of soybean isoflavones by regulating the expression of GmMYB176.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse phase

PROVIDER: MTBLS12627 | MetaboLights | 2025-06-23

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS12627_LC-MS_alternating_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS12627_LC-MS_alternating_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS12627.txt	Txt

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