Subcutaneous white adipose tissue-derived extracellular vesicle maintains intestinal homeostasis via IgA biosynthesis in aging mice

ABSTRACT:

To identify the potential iWAT-derived adipokines that regulate intestinal IgA biosynthesis, we conducted untargeted metabolomics in the serum of iFR and iSham mice. A total of 214 differential abundant metabolites were identified (log2FC >1 or <-1 and adjusted p-value <0.05) between the two groups. Pathway analysis showed that retinol metabolism was the most significantly altered pathway by iWAT removal. Despite retinol level remained unchanged, its downstream oxidized products, including retinal, RA, and all-trans-5,6-epoxy-RA, were significantly downregulated in the iFR mice. The reduction of RAs was further confirmed through using a targeted LC-MS/MS analysis. 

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12800 | MetaboLights | 2025-09-05

REPOSITORIES: MetaboLights