Dataset Information

Sericin Enhances Cryopreserved Sperm Quality in Chengde Hornless Black Goats by increasing Glutamine metabolism

ABSTRACT:

Cryopreservation of Chengde polled goat semen plays a critical role in conserving genetic resources, enhancing the utilization efficiency of superior breeding rams, and advancing artificial insemination techniques. However, spermatozoa are vulnerable to oxidative stress during the freezing process, which can significantly compromise sperm motility. This study demonstrated that supplementation of the semen cryoprotectant with 0.6% sericin significantly improved post-thaw sperm viability to 65.25% in Chengde hornless goats, while concurrently reducing both the sperm abnormality rate and intracellular ROS levels. Integrated TMT proteomics and LC/MS metabolomics identified 256 differentially expressed proteins and 109 differential metabolites between sericin supplementation and frozen control groups. Functional analysis revealed significant enrichment of differential metabolites, such as glutamine, in the alanine, aspartate, and glutamate metabolism pathway, concomitant with marked upregulation of antioxidant proteins including LRP8, GSTM3, and SIRT2. Thus, sericin enhances cryotolerance primarily by improving sperm viability, reducing oxidative damage, and sustaining energy metabolism. These findings establish the molecular foundation for optimizing goat semen cryopreservation and advancing artificial insemination technology.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS12860 | MetaboLights | 2025-08-15

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS12860_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS12860_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS12860_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS12860_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:<p>Enhancing the nation's beef supply and guaranteeing long-term food security requires increasing the effectiveness of artificial insemination (AI) in Bali cattle (Bos javanicus). Cryotolerance, or post-thaw variability in semen quality, limits AI results and probably reflects systemic and cellular metabolic variations. To determine the metabolic determinants of cryotolerance under standardized nutrition and supervision, this study combined blood biochemistry with the sperm metabolome. Ten healthy breeding bulls at the regional artificial insemination center (RAIC) were maintained on a uniform forage–concentrate diet. Blood biochemical parameters were measured using ethylenediaminetetraacetic acid (EDTA) plasma. For metabolomics, washed sperm pellets were prepared from frozen–thawed semen straws and profiled using untargeted gas chromatography-mass–mass spectrometry (GC–MS). Multivariate analyses (hierarchical clustering, K-means, and partial least squares–discriminant analysis (PLS-DA) with variable importance in projection (VIP) scores) summarized the global patterns. Spearman correlations were used to integrate blood indices, sperm metabolites, and post-thaw traits (motility, viability, plasma membrane integrity (%PMI), and morphological abnormalities). Eighteen intracellular metabolites were identified, predominated by fatty acyls. Unsupervised clustering and PLS-DA revealed clear inter-individual separation, with palmitic and stearic acids being among the most discriminant features (VIP ≥ 1.0). Systemic markers of lipid carriage and ionic tone aligned with sperm lipid composition: albumin and potassium were associated with higher intracellular palmitate levels and related metabolites. Functionally, lipid and short-chain fatty acid features, 1-monopalmitin, nonadecanoic acid, caproic acid, and valeric acid, were positively associated with viability and/or PMI %, whereas dodecanoic acid and glycerol monostearate were inversely related to morphological abnormalities. However, no robust association was detected with motility. Under a controlled dietary baseline, a lipid-centric blood-to-sperm metabolic axis emerges as a key determinant of cryotolerance in B. javanicus. The prioritized metabolites constitute practical biomarker candidates for sire screening and provide a mechanistic basis for refining extenders and cryopreservation protocols at AI centers. Targeted tandem mass spectrometry, membrane-focused lipidomics, and mitochondrial functional assays offer immediate paths to translation, with the potential to improve reproductive efficiency and, ultimately, bolster sustainable beef production and food security.</p>

Dataset Information

Sericin Enhances Cryopreserved Sperm Quality in Chengde Hornless Black Goats by increasing Glutamine metabolism

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