Proteomics,Multiomics

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Evaluation by re-derivation of a paternal line after 18th generations on seminal traits, proteome and fertility: SWATH quantitation of Seminal Plasma proteins


ABSTRACT: Breeding schemes for meat production in rabbits involved a three-way cross of specialized lines in which a paternal line inseminates maternal crossbred females. Paternal line or terminal sire are selected for growth traits, being the males used for the production of fertile dose at the insemination centres and farms. So high growth rate males must produce, in addition, semen in sufficient quantity and quality to meet the demand of insemination and, nevertheless, several studies have been showed that selection for growth have effects on reproduction performance in female and males. In rabbits, negative effects has been observed in ovulation induction, prenatal survival and genetic correlation to fertility. Many factors influence the production and quality of rabbit semen, management as collection frequency, environment (season or photoperiod, nutrition and genetic. Most of the previous studies have been focused in the effects of selection on the seminal and sperm parameters but, little attention has been paid to the protein seminal plasma or sperm composition and if these changes could be affect the fertility of seminal doses obtained from the paternal males. The aim of this study was to evaluate if selection program by daily gain in fattening period has changed seminal traits, plasma and sperm proteoma and, the fertility of semen when it is used in artificial insemination. To do this we uses two re-derived groups of paternal males obtained from vitrified embryos with a difference of 18 generations between both groups.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Seminal Plasma

DISEASE(S): Infertility

SUBMITTER: Luz Valero  

LAB HEAD: Francisco Marco Jimenez

PROVIDER: PXD015517 | Pride | 2020-01-13

REPOSITORIES: Pride

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| E-GEOD-57351 | biostudies-arrayexpress