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Proteomics and metabolomics analysis based on exosomes revealed the response mechanism of Alicyclobacillus acidoterrestris to acid stress

ABSTRACT:

Bacteria secrete exosomes containing stress-related molecules such as proteins and nucleic acids, which facilitate adaptation to challenging environments. In order to elucidate the role of A. acidoterrestris exosomes in its acid stress response, this study combined proteomics and metabolomics to analyze the function and biological pathway of exosomes in A. acidoterrestris against acid stress The results of exosome proteomics showed that spore coat protein D, SecA, YidC, chemotactic protein CheA, methyl-accepting chemotactic protein (MCP), serine-type D-alanine-D-alanine carboxypeptidase protein, and lipid II glutamine synthetase subunit GatD protein were significantly up-regulated. These proteins are mainly enriched in ABC transporters, two-component systems, quorum sensing, peptidoglycan biosynthesis and other pathways. The results of exosomal metabolomics showed that 83 and 68 differential metabolites were detected in positive and positive ion modes, respectively. These metabolites were mainly enriched in ABC transporters, carbohydrate metabolism and amino acid metabolism. This study showed that the exosome proteins and metabolites secreted by A. acidoterrestris under acid stress changed significantly, revealing the overall response of A. acidoterrestris to acid stress, and pointing out the possible future direction for the effective control and application of A. acidoterrestris

INSTRUMENT(S): Liquid Chromatography MS - positive - HILIC

PROVIDER: MTBLS12995 | MetaboLights | 2025-09-13

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS12995_LC-MS_positive_hilic_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS12995_LC-MS_positive_hilic_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS12995.txt	Txt

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Project description:Background: An excess of exosomes, nanovesicles released from all cells and key regulators of brain plasticity, is an emerging therapeutic target for stress-related mental illnesses. The effects of chronic stress on exosome levels are unknown; even less is known about molecular drivers of exosome levels in the stress response. Methods: We used our state-of-the-art protocol with two complementary strategies to isolate neuronal exosomes from plasma, ventral dentate gyrus, basolateral amygdala, and olfactory bulbs of male mice to determine the effects of chronic restraint stress (CRS) on exosome levels. Next, we used RNA sequencing and bioinformatic analyses to identify molecular drivers of exosome levels. Results: We found that CRS leads to an increase in the levels of neuronal exosomes but not total (i.e., not neuronally enriched) exosome levels assayed in plasma and the ventral dentate gyrus, whereas CRS leads to a decrease in neuronal exosome levels but not total exosome levels in the basolateral amygdala. There was a further specificity of effects as shown by a lack of changes in the levels of neuronal exosomes assayed in the olfactory bulbs. In pursuit of advancing translational applications, we showed that acetyl-L-carnitine administration restores the CRS-induced increase in neuronal exosome levels assayed in plasma (the most accessible specimen). Furthermore, the CRS-induced changes in neuronal exosome levels in the ventral dentate gyrus and basolateral amygdala mirrored the opposite pattern of CRS-induced transcriptional changes in these key brain areas, with β-estradiol signaling as a potential upstream driver of neuronal exosome levels. Conclusions: This study provides a foundation for future studies of new forms of local and distant communication in stress neurobiology by demonstrating specific relationships between neuronal exosome levels assayed in plasma and the brain and providing new candidate targets for the normalization of exosome levels.

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Proteomics and metabolomics analysis based on exosomes revealed the response mechanism of Alicyclobacillus acidoterrestris to acid stress

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