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A microfluidic chip recapitulating the human neurovascular unit with a functional blood-brain barrier for modeling gut-brain interactions

ABSTRACT:

The blood-brain barrier (BBB) is essential for central nervous system homeostasis, but most current in vitro models lack structural and functional fidelity. We developed a physiologically relevant human neurovascular unit microfluidic chip (hNVU-on-a-chip) incorporating brain microvascular endothelial cells, astrocytes, and microglia to reconstruct a biomimetic BBB microenvironment. Barrier function was confirmed by low apparent permeability (Papp) and active P-glycoprotein (P-gp) efflux, with performance superior to Transwell models. Transcriptomic profiling revealed endothelial maturation with upregulated barrier and transport genes and downregulated proliferative pathways. Introducing gut microbial metabolites altered brain-side neurotransmitter metabolism, elevating biogenic amines and reducing precursors, consistent with enhanced turnover. Together, the hNVU-on-a-chip recapitulates BBB architecture and function, and provides a robust platform to investigate gut-brain axis interactions.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse phase

PROVIDER: MTBLS13230 | MetaboLights | 2025-10-28

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS13230_LC-MS_alternating_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13230_LC-MS_alternating_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS13230.txt	Txt

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