Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A microfluidic chip recapitulating the human neurovascular unit with a functional blood-brain barrier for modeling gut-brain interactions

ABSTRACT:

The blood-brain barrier (BBB) is essential for central nervous system homeostasis, but most current in vitro models lack structural and functional fidelity. We developed a physiologically relevant human neurovascular unit microfluidic chip (hNVU-on-a-chip) incorporating brain microvascular endothelial cells, astrocytes, and microglia to reconstruct a biomimetic BBB microenvironment. Barrier function was confirmed by low apparent permeability (Papp) and active P-glycoprotein (P-gp) efflux, with performance superior to Transwell models. Transcriptomic profiling revealed endothelial maturation with upregulated barrier and transport genes and downregulated proliferative pathways. Introducing gut microbial metabolites altered brain-side neurotransmitter metabolism, elevating biogenic amines and reducing precursors, consistent with enhanced turnover. Together, the hNVU-on-a-chip recapitulates BBB architecture and function, and provides a robust platform to investigate gut-brain axis interactions.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse phase

PROVIDER: MTBLS13230 | MetaboLights | 2025-10-28

REPOSITORIES: MetaboLights

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	a_MTBLS13230_LC-MS_alternating_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13230_LC-MS_alternating_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS13230.txt	Txt

Items per page:

1 - 4 of 4

Similar Datasets

Transcriptome analysis of 3D model of blood-brain barrier exposed to Borrelia garini

Project description:Using RNA sequencing to map differentially expressed genes in the 3D model of the blood-brain barrier ( composed of human brain endothelial cells, human astrocytes, and human pericytes) challenged with Borrelia garini .

2021-01-01 | E-MTAB-9753 | biostudies-arrayexpress

Transcriptome analysis of 3D model of blood-brain barrier exposed to West Nile virus (WNV)

2021-01-01 | E-MTAB-9754 | biostudies-arrayexpress

Transcriptome analysis of 3D model of blood-brain barrier exposed to tick-borne encephalitis virus (TBEV)

2021-01-01 | E-MTAB-9755 | biostudies-arrayexpress

Transcriptomic profiling of a human iPSC-derived blood-brain barrier-on-chip model of ischemic stroke

Project description:Ischemic stroke is a leading cause of death and disability worldwide, characterized by reduced cerebral perfusion and blood-brain barrier (BBB) disruption. To model the human-specific pathophysiology of ischemic stroke, we developed a fully iPSC-derived blood-brain barrier-on-chip (iBBB-on-chip) comprising brain microvascular endothelial cells (iBMECs), pericytes (iPericytes), and astrocytes (iAstrocytes). After establishing a functional BBB structure, we induced ischemic injury by exposing the chip to oxygen-glucose deprivation (OGD) under static conditions. Transcriptome profiling of brain side ande endothelial side was performed to investigate molecular responses to ischemic stress.

2026-04-15 | GSE294722 | GEO

Modular Tissue-in-a-CUBE platform to model blood-brain barrier (BBB) and brain interaction

Project description:With the advent of increasingly sophisticated organoids, there is growing demand for technology to replicate the interactions between multiple tissues or organs. This is challenging to achieve, however, due to the varying culture conditions of the different cell types that make up each tissue. Current methods often require complicated microfluidic setups, but fragile tissue samples tend not to fare well with rough handling. Furthermore, the more complicated the human system to be replicated, the more difficult the model becomes to operate. Here, we present the development of a multi-tissue chip platform that takes advantage of the modularity and convenient handling ability of a CUBE device. We first developed a blood-brain barrier-in-a-CUBE by layering astrocytes, pericytes, and brain microvascular endothelial cells in the CUBE, and confirmed the expression and function of important tight junction and transporter proteins in the blood-brain barrier model. Then, we demonstrated the application of integrating Tissue-in-a-CUBE with a chip in simulating the in vitro testing of the permeability of a drug through the blood-brain barrier to the brain and its effect on treating the glioblastoma brain cancer model. We anticipate that this platform can be adapted for use with organoids to build complex human systems in vitro by the combination of multiple simple CUBE units.

2024-01-18 | GSE253222 | GEO

Single-cell RNA sequencing of a 3D blood-brain-barrier model perfused with P. falciparum-infected red blood cells and egress products

Project description:This dataset consists of two individual sample-multiplexing (MULTI-seq) single-cell RNA sequencing experiments, MB10x01 and MB10x02. Single-cell RNA sequencing (10X Genomics) analyses were performed on a microfluidic 3D in vitro blood-brain-barrier model (containing primary human brain microvascular endothelial cells, brain vascular pericytes, and astrocytes) perfused with P. falciparum egress product (MB10x01) or P. falciparum-infected red blood cells (RBC) (MB10x02). Dataset MB10x01 included two samples multiplexed by MULTI-seq sample barcoding (TCCTCGAA for control RBC lysate, ATGCGATG for P. falciparum egress product). P. falciparum egress product was obtained by letting tightly synchronized P. falciparum-infected RBC egress in media used for perfusions (5x10^7 infected RBC/ml). 3D blood-brain-barrier models perfused with P. falciparum egress products were incubated for 24 hours and compared to a control perfused with uninfected red blood cell lysate. MULTI-seq barcoding (McGinnis et al.Ê2019) was used for sample-barcoding of these two conditions, and the dataset contains cDNA (transcriptome) and sample barcode read files. Dataset MB10x02 included three samples multiplexed by MULTI-seq sample barcoding (GCTATGCA for control RBC, CGATACTG for Trophozoite stage, TACGCAGT for Schizont stage). 3D blood-brain-barrier models were perfused for 30 minutes with P. falciparum-infected RBC in the Trophozoite stage (26-34 hours post invasion) or Schizont stage (42-48 hours post invasion) (5x10^7 infected RBC/ml). After a 20-minute wash, the 3D blood-brain-barrier models were incubated with the bound P. falciparum-infected RBC for 6 hours and compared to uninfected RBC perfused controls. MULTI-seq barcoding was used for sample-barcoding of the three conditions, and the dataset contains cDNA (transcriptome) and sample barcode read files.

2025-06-25 | E-MTAB-14463 | biostudies-arrayexpress

Human neurovascular unit chip with a functional blood-brain barrier models brain-infection mechanism of a fungal meningitis pathogen

Project description:Reconstitution of a neurovascular unit model with blood-brain barrier (BBB) function is of great importance for drug development targeting cerebral diseases and study of brain disease mechanisms. In this study, we describe a human neurovascular unit chip designed by reconstituting necessary cellular and extracellular components in microfluidic devices. Dynamic three-dimensional co-culture of human cells (neural stem cells, brain microvascular endothelial cells, and brain vascular pericytes) in microfluidics with a stepwise unidirectional flow successfully established the chip with BBB-mimetic structural and functional features to be an effective barrier for drugs and cytokines. Furthermore, we showed the utility of the chip by modeling the neurotropic behaviors and BBB penetration process of a global fungal meningitis pathogen, Cryptococcus neoformans. This chip is the first in vitro experimental model for monitoring neurotropism of C. neoformans and would contribute to the investigation of various cerebral disorders, including microbial infectious diseases and their corresponding drug development.

2021-04-13 | GSE171937 | GEO

Generation of hiPSC-derived brain microvascular endothelial cells using a combination of directed differentiation and transcriptional reprogramming strategies

Project description:The blood-brain barrier (BBB), formed by specialized brain microvascular endothelial cells (BMECs), regulates brain function in health and disease. In vitro modeling of the human BBB is limited by the lack of robust protocols to generate BMECs from iPSCs. Here, we report generation of reprogrammed BMECs (rBMECs) through a combination of hiPSC differentiation into BBB-primed endothelial cells (bpECs) and subsequent reprogramming with two BBB transcription factors, FOXF2 and ZIC3. rBMECs express a subset of the BBB gene repertoire including tight junctions and transporters, exhibit higher paracellular barrier properties, and have lower tracer diffusion and transcellular transport compared to primary hBMECs, and can be activated by oligomeric Ab42 . We then generated an hiPSC 3D microfluidic system that incorporates rBMECs, pericytes and astrocytes using the MIMETAS platform. This novel 3D system closely resembles the in vivo BBB at structural, functional and transcriptome levels, and can be used to study pathogenic mechanisms of neurological diseases.

2025-12-31 | GSE267498 | GEO

Regulation of blood-brain barrier by astrocyte through mitochondrial transfer II

Project description:In our study, astrocytes were proven to be capable of delivering mitochondria to endothelial cells, and thus regulate the integrity of blood-brain barrier. We utilized RNA seq to investigate the effect of astrocyte-derived mitochondria on endothelial cells.

2024-07-31 | GSE206414 | GEO

A proteomics resource for human iPSC-derived endothelial cells (iEC), smooth muscle cells (iSMC), pericytes (iPC) and astrocytes (iAS) in comparison to human primary cells - technical and biological replicates

Project description:Integrity of the blood-brain barrier (BBB) is critical for brain homeostasis, and its malfunction contributes to neurovascular and neurodegenerative disorders. So far, mechanistic studies on BBB function have been mostly conducted in rodent and non-physiological in vitro models, which recapitulate some disease features, but have limited translatability to humans and pose challenges for drug discovery. Here we report on a fully human iPSC-derived, microfluidic 3D BBB model consisting of endothelial cells (EC), mural cells, and astrocytes. Our model expresses typical cell fate markers, forms a barrier in vessel-like tubes, and enables perfusion, including with human blood. We optimized iPSC differentiations and validated cellular fates by comparison to published datasets and extensive benchmarking vs. primary cells with proteomic profiles provided in an online database (https://dbNeuroVasP.isd-muc.de).

2025-09-08 | PXD066414 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data