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Transcriptomic profiling of a human iPSC-derived blood-brain barrier-on-chip model of ischemic stroke

ABSTRACT: Ischemic stroke is a leading cause of death and disability worldwide, characterized by reduced cerebral perfusion and blood-brain barrier (BBB) disruption. To model the human-specific pathophysiology of ischemic stroke, we developed a fully iPSC-derived blood-brain barrier-on-chip (iBBB-on-chip) comprising brain microvascular endothelial cells (iBMECs), pericytes (iPericytes), and astrocytes (iAstrocytes). After establishing a functional BBB structure, we induced ischemic injury by exposing the chip to oxygen-glucose deprivation (OGD) under static conditions. Transcriptome profiling of brain side ande endothelial side was performed to investigate molecular responses to ischemic stress.

ORGANISM(S): Homo sapiens

PROVIDER: GSE294722 | GEO | 2026/04/15

REPOSITORIES: GEO

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