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Multi-omics insights into high-carotenoid hybrid potato lines: enhanced nutritional quality and metabolic regulation for food applications

ABSTRACT:

Modern potato varieties often exhibit low carotenoid content, limiting their nutritional value and processing performance. This study employed a multi-omics approach (physicochemical analysis, metabolomics, mineral profiling, transcriptomics, and microbiome sequencing) to investigate the molecular basis of high-carotenoid biofortification in two elite hybrid potato lines (H4 and H14) compared with four commercial cultivars. H4 and H14 showed higher starch (beneficial for processing), zeaxanthin and lutein, elevated phenolic acids (vanillic and salicylic acid), optimal K/Ca, and reduced reducing sugars, improving storage. Key pathways in starch metabolism, carotenoid biosynthesis, and amino acid metabolism were reprogrammed, with β-carotene hydroxylase (BCH2) and transcription factors (MYB, bHLH, NAC, WRKY) regulating carotenoid accumulation. Microbiome analysis revealed enriched Bacillus and Lactobacillales in H4/H14, suggesting a role in metabolic support. These findings highlight metabolic and microbial mechanisms underlying high-carotenoid, high-nutrition, and processing-optimized potatoes, offering a strategy for biofortified cultivar development to enhance nutrition and sustainability.

INSTRUMENT(S): Liquid Chromatography MS - alternating - direct infusion

PROVIDER: MTBLS13298 | MetaboLights | 2025-11-12

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS13298_LC-MS_alternating_direct-infusion_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13298_LC-MS_alternating_direct-infusion_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS13298.txt	Txt

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