Integrated metabolomic and microbiomic analysis of the effects of dietary Candida utilis replacement of fishmeal on the growth, physiology, and intestinal microbiota of southern catfish (Silurus meridionalis)

ABSTRACT:

Liver samples of southern catfish were sent to Majorbio Bio-pharm Technology Co., Ltd. for untargeted metabolomic sequencing referring to previous study [27]. Grinding beads were added to the liver samples, and metabolites were extracted via low-temperature ultrasound using an extraction solution (methanol:water = 4:1, v/v) containing four internal standards (including L-2-chlorophenylalanine at 0.02 mg/mL). The samples were then incubated at -20℃ for 30 min, centrifuged at 13,000 g for 15 min at 4℃, and the supernatant was transferred to injection vials with inner liners for instrumental analysis. Quality control (QC) samples were prepared by mixing equal volumes of metabolites from all samples, and one QC sample was inserted every 5–10 samples during instrumental analysis to evaluate the repeatability of the entire analytical process. LC-MS analysis was performed on a Thermo Fisher Scientific ultra-high-performance liquid chromatography-tandem Fourier transform mass spectrometry (UHPLC-Orbitrap Exploris 240) system. After instrumental analysis, the raw LC-MS data were imported into the metabolomics processing software Progenesis QI (Waters Corporation, Milford, USA) for baseline filtering, peak detection, integration, retention time correction, and peak alignment. Finally, a data matrix including retention time, mass-to-charge ratio (m/z), and peak intensity was obtained. Meanwhile, LC-MS spectral information was matched against public metabolomic databases (HMDB: http://www.hmdb.ca/; Metlin: https://metlin.scripps.edu/) and Majorbio’s in-house database to acquire metabolite information.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS13346 | MetaboLights | 2025-11-19

REPOSITORIES: MetaboLights