Dataset Information

Comparative Transcriptome and Energy Metabolome Profiling Unravel the Key Role of GlcN6P Metabolic Pathway in Resistance against Bacteriophage in Enterotoxigenic Escherichia coli

ABSTRACT:

The rapid emergence of phage-resistant bacterial mutants is a major challenge for phage application in the food industry. The resistance mechanisms of Enterotoxigenic E. coli (ETEC) against phage infection are largely unknown. In Escherichia coli, RapZ regulates glucosamine-6-phosphate (GlcN6P) metabolism, the formation of which initiates synthesis of the bacterial cell envelope, including lipopolysaccharides (LPS). Previously, RapZ mediated phage-resistant mutants WRP and rapZE227Stop were identified in a phage aerosol spray assay. In this study, comparative transcriptomic and energy metabolomic analyses showed that the differentially expressed genes and differentially accumulated metabolites from WRP and rapZE227Stop were mostly enriched in metabolic pathways, mainly in GlcN6P biosynthesis. Additionally, GlcN6P biosynthesis-related gene expression was significantly upregulated or downregulated in phage-resistant mutants compared to that in wild type (WT). Some metabolites involved in GlcN6P metabolic pathways, such as GlcN6P, GlcNAc-6P, GlcNAc-1P and UDP-GlcNAc were upregulated in phage-resistant mutants. Furthermore, the reduction in LPS content and the resensitization to antibiotics reveal the important role of the GlcN6P metabolic pathway in RapZ mediated phage resistance. These results suggest that GlcN6P metabolic pathways play important roles in ETEC resistance to phage infection and provide useful insights for developing phage based applications.

INSTRUMENT(S): Liquid Chromatography MS - alternating - hilic, Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS13411 | MetaboLights | 2025-12-15

REPOSITORIES: MetaboLights

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	a_MTBLS13411_LC-MS_alternating_hilic_metabolite_profiling.txt	Txt
	a_MTBLS13411_LC-MS_alternating_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13411_LC-MS_alternating_hilic_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS13411_LC-MS_alternating_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

Items per page:

1 - 5 of 6

Similar Datasets

Project description:<p>Traveler's diarrhea (TD) is caused by enterotoxigenic Escherichia coli (ETEC), other pathogenic gram-negative pathogens, norovirus and some parasites. Nevertheless, standard diagnostic methods fail to identify pathogens in more than 30% of TD patients, so it is predicted that new pathogens or groups of pathogens may be causative agents of disease. A comprehensive metagenomic study of the fecal microbiomes from 23 TD patients and seven healthy travelers was performed, all of which tested negative for the known etiologic agents of TD in standard tests. Metagenomic reads were assembled and the resulting contigs were subjected to semi-manual binning to assemble independent genomes from metagenomic pools. Taxonomic and functional annotations were conducted to assist identification of putative pathogens. We extracted 560 draft genomes, 320 of which were complete enough to be enough characterized as cellular genomes and 160 of which were bacteriophage genomes. We made predictions of the etiology of disease in individual subjects based on the properties and features of the recovered cellular genomes. Three subtypes of samples were observed. First were four patients with low diversity metagenomes that were predominated by one or more pathogenic E. coli strains. Annotation allowed prediction of pathogenic type in most cases. Second, five patients were co-infected with E. coli and other members of the Enterobacteriaceae, including antibiotic resistant Enterobacter, Klebsiella, and Citrobacter. Finally, several samples contained genomes that represented dark matter. In one of these samples we identified a TM7 genome that phylogenetically clustered with a strain isolated from wastewater and carries genes encoding potential virulence factors. We also observed a very high proportion of bacteriophage reads in some samples. The relative abundance of phage was significantly higher in healthy travelers when compared to TD patients. Our results highlight that assembly-based analysis revealed that diarrhea is often polymicrobial and includes members of the Enterobacteriaceae not normally associated with TD and have implicated a new member of the TM7 phylum as a potential player in diarrheal disease. </p>

Dataset Information

Comparative Transcriptome and Energy Metabolome Profiling Unravel the Key Role of GlcN6P Metabolic Pathway in Resistance against Bacteriophage in Enterotoxigenic Escherichia coli

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets