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Metabolomic signatures in blastocyst spent culture medium as noninvasive predictors of live birth: a pilot study

ABSTRACT:

Research Question: Can metabolomic profiling of blastocyst spent culture medium (SCM) serve as a non-invasive tool to predict live birth?

Design: This pilot study investigated the association between metabolite profiles in SCM and clinical outcomes following single blastocyst transfer. Seventy SCM samples were analyzed using untargeted liquid chromatography-mass spectrometry (LC-MS). Metabolites were identified via Human Metabolome Database (HMDB) and Compound Discoverer 3.3. Statistical analyses were performed using SPSS.

Results: Six metabolites exhibited significant differences between pregnancy and non-pregnancy groups, while ten metabolites varied between the live birth and non-live birth groups. Notably eicosapentaenoic acid (EPA), a polyunsaturated fatty acid, decreased in pregnancy cases. In contrast 1-(4-methoxyphenyl)- 3-pentanone and (2S)-2-(6-methoxynaphthalen-2-yl) propanoic acid were consistently elevated in both pregnancy and live birth groups. Additional differential metabolites included L-glutamine, pyroglutamylglycine, alanylproline, and 15,16-dihydroxyoctadecanoic acid, potentially reflecting implantation-related metabolic activity. Logistic regression and receiver operating characteristic (ROC) curve analyses demonstrated acceptable predictive performance, with an area under curve (AUC) values of 0.788 for pregnancy and 0.834 for live birth.

Conclusions: Metabolomic profiling of SCM may offer a promising noninvasive adjunct to embryo selection strategies. While these findings suggest biological relevance of several metabolites, particularly lipids and amino acid derivatives, larger studies are needed to validate predictive value and clinical applicability.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS13898 | MetaboLights | 2026-02-13

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS13898_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS13898_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13898_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS13898_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:Preterm birth is a main determinant of neonatal mortality and morbidity and a major contributor to the overall mortality and burden of disease. However, precise phenotyping of the preterm birth is hampered by the imprecise definition of the clinical phenotype and complexity of the molecular phenotype due to multiple pregnancy tissue types and molecular processes that may contribute to the preterm birth. The studyâ??s aim was to comprehensively evaluate the mRNA transcriptome that characterizes preterm and term labor using precisely phenotyped samples. Expression profiles of 73 genes and non-coding RNA sequences uniquely identified the four groups of patients: delivering preterm with (PL) and without labor (PNL), term with (TL) and without labor (TNL). The largest differences in gene expression among the four groups occurred in decidua, chorion and amnion. The gene expression profiles showed suppression of chemokines expression in TNL, withdrawal of this suppression in TL, activation of multiple pathways of inflammation in PL, and an immune rejection profile in PNL. The genes constituting expression signatures showed over-representation of three putative regulatory elements in their 5â?? and 3â??UTR regions. The results suggest that pregnancy is maintained by downregulation of chemokines at the maternal-fetal interface. Withdrawal of this downregulation results in the term birth and its overriding by the activation of multiple pathways of the immune system in the preterm birth. Complications of the pregnancy associated with impairment of placental function, which necessitated premature delivery of the fetus in the absence of labor, show gene expression patterns associated with immune rejection. 183 total RNA samples from 8 tissue types collected from 35 women grouped into six categories of pregnancy outcome. One microarray replicate per sample. Other Contributors: Radek Bukowski, Sam Parry and the NICHD Genomic and Proteomic Network for Preterm Birth Research

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Metabolomic signatures in blastocyst spent culture medium as noninvasive predictors of live birth: a pilot study

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