Dataset Information

Mechanism of Organic Plant Protection Technology in Controlling Apple Bitter Pit: Insights from Integrated Transcriptomic and Metabolomic Analyses

ABSTRACT:

Apple bitter pit (ABP), a severe physiological disorder, severely impairs the quality and yield of the susceptible 'Qingcui' apple. This study investigated the control efficacy and mechanism of a three-in-one Organic Plant Protection Technology (OPPT) against ABP, with treatments set as control (CK), untreated diseased (UD), and OPPT-sprayed healthy groups. Results showed OPPT significantly reduced ABP incidence to 14.84%, improved fruit quality, enhanced antioxidant defense systems, mitigated oxidative damage, repaired fruit cell structural damage, and restored fruit calcium levels to the healthy level. Integrated transcriptomic and metabolomic analyses revealed OPPT reshaped fruit gene and metabolite profiles, with the flavonoid biosynthesis pathway as the core regulatory node, upregulating key genes (CHS, FLS, etc.) and promoting flavonoid metabolite accumulation. In conclusion, OPPT controls ABP via multi-dimensional regulation of phenotype, physiology, cell structure and molecular metabolism, providing a novel paradigm for the green control of apple physiological disorders and supporting sustainable apple industry development.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS13937 | MetaboLights | 2026-02-26

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	a_MTBLS13937_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS13937_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS13937_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS13937_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

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Project description:Molecular events regulating apple fruit ripening and sensory quality are largely unknown. Such knowledge is essential for genomic-assisted apple breeding and postharvest quality management. In this study, a parallel transcriptome profile analysis, scanning electron microscopic (SEM) examination and systematic physiological characterization were performed on two apple cultivars, Honeycrisp (HC) and Cripps Pink (CP), which have distinct ripening features and texture attributes. Systematic physiological characterization of fruit ripening based on weekly maturity data indicated substantial differences in fruit crispness and firmness at comparable ripening stages. SEM images of fruit cortex tissues prepared from fruits with equivalent maturity suggested that the cell wall thickness may contribute to the observed phenotypes of fruit firmness and crispness. A high-density long-oligo apple microarray consisting of duplex 190,135 cross-hybridization-free 50-70-mer isothermal probes, and representing 23,997 UniGene clusters, was manufactured on a Nimblegen array platform. Transcriptome profiling identified a total of 1793 and 1209 UniGene clusters differentially expressed during ripening from cortex tissues of HC and CP, respectively. UniGenes implicated in hormone metabolism and response, cell wall biosynthesis and modification and those encoding transcription factors were among the prominent functional groups. Between the two cultivars, most of the identified UniGenes were similarly regulated during fruit ripening; however, a short list of gene families or specific family members exhibited distinct expression patterns between the two cultivars, which may represent candidate genes regulating cultivar-specific apple fruit ripening patterns and quality attributes. Using a single color labeling system, a total of 24 microarray slides were utilized, one for each cortex tissue sample, for transcriptome profiling analysis. 2 cultivars x 3 developmental stages x 4 biological replicates.

Dataset Information

Mechanism of Organic Plant Protection Technology in Controlling Apple Bitter Pit: Insights from Integrated Transcriptomic and Metabolomic Analyses

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