Dataset Information

Clostridium butyricum ameliorates meat quality in coccidiosis-affected sheep by rescuing thiamine diphosphate-mediated glucose metabolism

ABSTRACT:

Sheep coccidiosis, caused by Eimeria spp., is widely studied for inducing diarrhea, but its impact on mutton quality remains poorly understood. Here, we systematically investigated the effects of Eimeria infection on meat quality and the interventing role of Clostridium butyricum (C. butyricum), alongside underlying mechanisms. Results showed that C. butyricum intervention (CB-IF group) significantly improved muscle pH and water-holding capacity, and textural properties (shear force, hardness; all p < 0.05). Eimeria infection reduced serum pro-inflammatory factors (IL-1β, IL-6, IL-18, and TNF-α) and the GSH/GSSG ratio, accompanied by an elevation in the anti-inflammatory factor IL-10. Meanwhile, local immunity (IL-17 and IL-18) and catalase activity in the Longissimus dorsi were suppressed, and these impairments were partially reversed by C. butyricum intervention. Integrative metabolomics and transcriptomics revealed that Eimeria spp. infection (IF group) downregulated thiamine transporter protein SLC19A1, reducing HET-P and thiamine diphosphate (TDP) levels in Longissimus dorsi, thereby disrupting glucose metabolism pathways, including the pentose phosphate pathway, fructose and mannose metabolism, galactose metabolism. The joint analysis indicated that Longissimus dorsi muscle compensated by upregulating glycosylation-related genes (GMPPB and TSTA3) and mitochondrial oxidative phosphorylation genes (NDUFA3, NDUFA11, NDUFB11, and COX17), leading to enhanced oxidative stress. Notably, C. butyricum intervention significantly upregulated SLC19A1 expression, restored TDP/HET-P levels, rebalanced energy metabolism, and improved meat qualtiy (e.g., elevated 24h pH and redness [a*]). This study reveals a novel mechanism through which thiamine diphosphate-mediated glucose metabolism modulates meat quality in sheep infected with Eimeria spp, thereby laying a theoretical foundation for formulating targeted nutritional strategies via probiotic intervention.

INSTRUMENT(S): Liquid Chromatography MS - positive, Liquid Chromatography MS - negative

PROVIDER: MTBLS14093 | MetaboLights | 2026-04-27

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS14093_LC-MS_negative__metabolite_profiling.txt	Txt
	a_MTBLS14093_LC-MS_positive__metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14093_LC-MS_negative__metabolite_profiling_v2_maf.tsv	Tabular
	m_MTBLS14093_LC-MS_positive__metabolite_profiling_v2_maf.tsv	Tabular

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Clostridium butyricum ameliorates meat quality in coccidiosis-affected sheep by rescuing thiamine diphosphate-mediated glucose metabolism

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