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Ultrasound Priming Enhances Seed Vigor and Multiple Stresses Tolerance in Aged Maize Seeds

ABSTRACT:

Seed aging leads to reduced germination rates and vigor, particularly under stress conditions. We subjected aged maize seeds to 40 seconds of ultrasonic treatment, which enhanced their vigor and tolerance to combined stress. The ultrasonic treatment increased more cracks and holes in the seed coat, thereby promoting water absorption. Concurrently, it improved the germination and root growth of aged seeds under combined stress, with enhancements observed in endogenous hormone levels, antioxidant enzyme activity, osmotic regulators, and hydrolase content. Under combined stress, ultrasound activated transcriptional and metabolic reprogramming in aged seeds, not only triggering more pathways associated with growth and defense but also activating the MAPK signaling pathway. Through structural, physiological and molecular reprogramming, ultrasound treatment enhanced the vigor and stress tolerance of aged seeds, providing an effective strategy for revitalizing stored seeds and coping with multiple stresses under adverse conditions.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS14138 | MetaboLights | 2026-03-25

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS14138_LC-MS_alternating_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14138_LC-MS_alternating_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular
	s_MTBLS14138.txt	Txt

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Project description:Background: Waterlogging was one of the most serious abiotic stresses in wheat-growing regions of China. There were great differences in waterlogging tolerance among different wheat varieties, and the mechanism of waterlogging tolerance of wheat seeds during germination was unclear. Methods: In order to reveal the adaptability of wheat to waterlogging stress during germination, we analyzed the germination rate and anatomical structure of three wheat seeds, ‘Zhoumai 22’, ‘Bainong 207’ and ‘Bainong 607’. At the same time, Illumina sequencing technology was used to determine the transcriptome of these three wheat varieties during germination. Results: The results showed that there was no significant difference between the germination rate of ‘Bainong 207’ after 3 days of waterlogging treatment and that of the control seeds. However, under waterlogging stress, the degree of emulsification and degradation of endosperm cells was higher than that of the control treatment, and starch granules in endosperm were significantly reduced. Transcriptome data were obtained from seed samples (a total of 18 samples) of three wheat varieties under waterlogging and control treatment. A total of 2,775 differentially expressed genes (DEGs) were identified by comprehensive analysis. In addition, by analyzing the correlation between the expression levels of DEGs and seed germination rates in three wheat varieties under waterlogging stress, it was found that the relative expression levels of 563 and 398 genes were positively and negatively correlated with the germination rate of wheat seeds, respectively. The GO and KEGG analysis found that the difference in waterlogging tolerance of the three wheat varieties was related to the abundance of key genes involved in the glycolysis pathway, the starch and sucrose metabolism pathway, and the lactose metabolism pathway. The ethanol dehydrogenase (ADH) gene in the endosperm of ‘Bainong 607’ was immediately induced after a short period of waterlogging, and the energy provided by glycolysis pathway enabled the seeds of ‘Bainong 607’ to germinate as early as possible, while the expression level of AP2/ERF transcription factor was up-regulated to further enhance its waterlogging tolerance. Conclusions: In general, this study provided a deeper understanding of the mechanisms by which different wheat varieties respond to waterlogging stress during germination.

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Ultrasound Priming Enhances Seed Vigor and Multiple Stresses Tolerance in Aged Maize Seeds

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