Metabolome of Streptomyces sp. HU2014
Ontology highlight
ABSTRACT: HU2014 was inoculated into an optimized medium containing dextrin (39.59 g/L), yeast extract (8.52 g/L), and KNO3 (2.49 g/L), with the initial pH adjusted to 7.0–7.3, and fermented at 25°C and 120 rpm for 15 days. Fermentation supernatants were collected at four time points: day 0 (C0/control), day 5 (T1), day 10 (T2), and day 15 (T3), with six biological replicates for each time point. Samples were filtered through 0.2 µm membranes, immediately snap-frozen in liquid nitrogen, and stored for subsequent analysis.
INSTRUMENT(S): Liquid Chromatography MS - alternating - normal-phase
PROVIDER: MTBLS14352 | MetaboLights | 2026-04-22
REPOSITORIES: MetaboLights
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| a_MTBLS14352_LC-MS_alternating_normal-phase.txt | Txt | |||
| i_Investigation.txt | Txt | |||
| m_MTBLS14352_LC-MS_alternating_normal-phase_v2_maf.tsv | Tabular | |||
| s_MTBLS14352.txt | Txt |
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Project description:Goal :<br>
Detection of unknown degradation products of the nitrification inhibitors DMPP (SNI) and MBOA (BNI) in three different soils (Wieselburg, Marchfeld, and
Linz).<br>
<br>
List of samples:<br>
Same labeling scheme for all three soils (L, MF, WB)<br>
Sample NR | Timepoint | Treatment | weighed soil [g] | Which medium is it stored in<br>
1 | T0 (same day) | DMPP | 0.25 | HPLC-eluent<br>
5 | T0 (same day) | DMPP | 0.25 | HPLC-eluent<br>
6 | T0 (same day) | DMPP - stable isotope | 0.25 | HPLC-eluent<br>
10 | T0 (same day) | DMPP - stable isotope | 0.25 | HPLC-eluent<br>
11 | T0 (same day) | MBOA | 0.25 | Acetonirile<br>
15 | T0 (same day) | MBOA | 0.25 | Acetonirile<br>
16 | T0 (same day) | MBOA - stable isotope | 0.25 | Acetonirile<br>
20 | T0 (same day) | MBOA - stable isotope | 0.25 | Acetonirile<br>
21 | T0 (same day) | Control | 0.25 | HPLC-eluent<br>
25 | T0 (same day) | Control | 0.25 | Acetonirile<br>
26 | T1 (after 3 days) | DMPP | 0.25 | HPLC-eluent<br>
30 | T1 (after 3 days) | DMPP | 0.25 | HPLC-eluent<br>
31 | T1 (after 3 days) | DMPP - stable isotope | 0.25 | HPLC-eluent<br>
35 | T1 (after 3 days) | DMPP - stable isotope | 0.25 | HPLC-eluent<br>
36 | T1 (after 3 days) | MBOA | 0.25 | Acetonirile<br>
40 | T1 (after 3 days) | MBOA | 0.25 | Acetonirile<br>
41 | T1 (after 3 days) | MBOA - stable isotope | 0.25 | Acetonirile<br>
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46 | T1 (after 3 days) | Control | 0.25 | Acetonirile<br>
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65 | T2 (after 10 days) | MBOA | 0.25 | Acetonirile<br>
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70 | T2 (after 10 days) | MBOA - stable isotope | 0.25 | Acetonirile<br>
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80 | T3 (after 14 days) | DMPP | 0.25 | HPLC-eluent<br>
81 | T3 (after 14 days) | DMPP - stable isotope | 0.25 | HPLC-eluent<br>
85 | T3 (after 14 days) | DMPP - stable isotope | 0.25 | HPLC-eluent<br>
86 | T3 (after 14 days) | MBOA | 0.25 | Acetonirile<br>
90 | T3 (after 14 days) | MBOA | 0.25 | Acetonirile<br>
91 | T3 (after 14 days) | MBOA - stable isotope | 0.25 | Acetonirile<br>
95 | T3 (after 14 days) | MBOA - stable isotope | 0.25 | Acetonirile<br>
96 | T3 (after 14 days) | Control | 0.25 | Acetonirile<br>
100 | T3 (after 14 days) | Control | 0.25 | HPLC-eluent<br>
<br>
Concentrations:<br>
all smaples have 130ug of (NH4)2SO4<br>
DMPP samples have 1,3ug of DMPP per 1 g soil<br>
MBOA samples have 300ug of MBOA per 1 g soil<br>
for samples that are isotopically labeled the amount of Inhibitor was split between the unlabeled and labeled compound<br>
5ul of the respective solution was added to the sample.<br>
<br>
Measurements according to 10.1021/ac503290j, but with an Q Exactive HF instrument<br>
<br>
Results: <br>
D patterns unfortunately also always show up in the T0 controls --> so it's unlikely to be biological breakdown/degradation products<br>
<br>
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