A targeted MRM-based lipidomic dataset of Tenebrio molitor larvae fed diets with different protein levels

ABSTRACT: Tenebrio molitor is increasingly used as a sustainable source of nutrients for food and feed production, yet their lipid metabolic responses to dietary protein remain poorly characterized at the molecular species level. Here, we report a targeted UPLC–MS/MS lipidomics dataset of Tenebrio molitor larvae fed four dietary treatments: a wheat bran control and three formulated diets containing 15%, 20% and 25% crude protein. The dataset includes 20 biological samples, with five independent replicates per treatment, each generated from pooled whole-body larvae. Using a scheduled multiple reaction monitoring method on a QTRAP 6500+ platform, 834 lipid species were annotated and quantified across major lipid categories, including glycerolipids, glycerophospholipids, glycolipids and sphingolipids. The data records comprise raw mzML files, processed concentration matrices, sample metadata, lipid annotation tables, MRM transition lists and quality-control metrics. Technical validation using pooled quality-control samples, coefficient-of-variation analysis, sample correlation, principal component analysis and hierarchical clustering supported the reproducibility of the dataset. This resource may facilitate studies of insect nutrition, dietary lipid remodeling, feed formulation and comparative lipidomics.

INSTRUMENT(S): Liquid Chromatography MS - negative - reversed-phase-c30, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14531 | MetaboLights | 2026-05-20

REPOSITORIES: MetaboLights