Untargeted Metabolomics of Mouse Platelet Pellets by LC-MS/MS

ABSTRACT: In this study, platelet pellets were isolated from mouse peripheral blood through gradient centrifugation to obtain high-purity platelet samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based untargeted metabolomics was performed to comprehensively profile the global metabolic changes and endogenous small-molecule metabolites in mouse platelets. All samples were subjected to strict pre-treatment, including metabolite extraction, protein removal, and chromatographic separation, to eliminate experimental interference and ensure the accuracy of mass spectrum detection. Subsequent bioinformatics analyses, including metabolite identification, differential metabolite screening, metabolic pathway enrichment, and principal component analysis, were conducted to screen distinct metabolic signatures and key enriched signaling pathways between different groups. This study aims to elucidate the metabolic characteristics of mouse platelets under specific conditions, identify potential functional metabolite biomarkers, and provide a reliable metabolic theoretical basis for exploring the physiological regulatory mechanisms and pathological roles of platelets in related diseases.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS15244 | MetaboLights | 2026-08-04

REPOSITORIES: MetaboLights