Targeted bile acid profiling of Faecalibaculum rodentium-induced bile acid remodeling in antibiotic-treated mice

ABSTRACT: This study investigated whether Faecalibaculum rodentium remodels the intestinal bile acid pool through bacterial bile salt hydrolase activity in antibiotic-treated male C57BL/6J mice. Mice were assigned to three groups: control, Faecalibaculum rodentium gavage, and Faecalibaculum rodentium gavage combined with the gut-restricted bile salt hydrolase inhibitor GR-7. Targeted bile acid profiling was performed on fecal samples using UPLC-ESI-MS/MS in negative-ion scheduled multiple reaction monitoring mode with an ExionLC AD system coupled to a SCIEX QTRAP 6500+ mass spectrometer. The deposited dataset contains 18 biological samples, with six samples per group, and quantitative measurements from a targeted panel of 82 bile acids.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase

PROVIDER: MTBLS15285 | MetaboLights | 2026-08-07

REPOSITORIES: MetaboLights