Integrated pharmacological and metabolomic characterization of a polysaccharide-enriched fraction from Qingfei-Paidu Decoction for protection against acute lung injury in mice

ABSTRACT: The formula of Qingfei-Paidu Decoction was separated into a polysaccharide-enriched fraction (PSC) and an ethanol-soluble small-molecule fraction (EtOH), whose biological activities were systematically compared under equivalent crude drug conditions using an LPS-induced ALI mouse model and macrophage inflammatory assays. PSC exhibited superior protective activity compared with EtOH, characterized by greater attenuation of pulmonary pathological injury, edema formation, and inflammatory cytokine production. Furthermore, 1H NMR-based serum metabolomics combined with shared-and-unique structures analysis revealed distinct metabolic fingerprints between the two fractions. Compared with EtOH, PSC produced broader metabolic remodeling, particularly involving energy metabolism.

INSTRUMENT(S): Nuclear Magnetic Resonance (NMR) -

PROVIDER: MTBLS15898 | MetaboLights | 2026-10-05

REPOSITORIES: MetaboLights