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Prokaryotic viperins produce diverse antiviral molecules

ABSTRACT: Viperin is an interferon-induced cellular protein conserved in animals. It was shown to inhibit the replication of multiple viruses by producing a ribonucleotide called 3’-deoxy-3’4’-didehydro-CTP (ddhCTP), which acts as a chain terminator for the viral RNA polymerase. Here we show that the eukaryotic viperin has originated from a clade of bacterial and archaeal proteins that protect against phage infection. Prokaryotic viperins (pVips) produce a set of modified ribonucleotides that include, in addition to ddhCTP, also ddhGTP and ddhUTP. We further provide evidence that pVips protect against T7 phage infection by inhibiting viral polymerase-dependent transcription, suggesting a conserved mechanism of action shared between pVips and the animal viperin. Our results unveil a potential repository of natural antiviral compounds produced by bacterial immune systems.

INSTRUMENT(S): Liquid Chromatography MS - positive, Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - negative - hilic

SUBMITTER: Aude Bernheim

PROVIDER: MTBLS1750 | MetaboLights | 2020-08-24

REPOSITORIES: MetaboLights

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	a_MTBLS1750_NEG_LC-MS2.txt	Txt
	a_MTBLS1750_POS_LC-MS.txt	Txt
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