Dataset Information

Comparative LC/MS-based profiling of silver nitrate-treated Arabidopsis thaliana leaves of wild-type and cyp79B2 cyp79B3 double knockout plants

ABSTRACT: Indole-3-acetaldoxime (IAOx) represents an early intermediate of the biosynthesis of a variety of indolic secondary metabolites including the phytoanticipin indol-3-ylmethyl glucosinolate and the phytoalexin camalexin (3-thiazol-2′-yl-indole). Arabidopsis thaliana cyp79B2 cyp79B3 double knockout plants are completely impaired in the conversion of tryptophan to indole-3-acetaldoxime and do not accumulate IAOx-derived metabolites any longer. Consequently, comparative analysis of wild-type and cyp79B2 cyp79B3 plant lines has the potential to explore the complete range of IAOx-derived indolic secondary metabolites.

INSTRUMENT(S): Liquid Chromatography MS - - - micrOTOF-Q II ESI TOF (Bruker)

PROVIDER: MTBLS2 | MetaboLights | 2012-05-22

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MSpos-Ex1-Col0-48h-Ag-1_1-A,1_01_9818.d.zip	Other
	SampleInfo.xml	Xml
	MSpos-Ex1-Col0-48h-Ag-2_1-A,1_01_9820.d.zip	Other
	SampleInfo.xml	Xml
	MSpos-Ex1-Col0-48h-Ag-3_1-A,1_01_9822.d.zip	Other

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Publications

The multifunctional enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) converts cysteine-indole-3-acetonitrile to camalexin in the indole-3-acetonitrile metabolic network of Arabidopsis thaliana.

Böttcher Christoph C Westphal Lore L Schmotz Constanze C Prade Elke E Scheel Dierk D Glawischnig Erich E

The Plant cell 20090630 6

Accumulation of camalexin, the characteristic phytoalexin of Arabidopsis thaliana, is induced by a great variety of plant pathogens. It is derived from Trp, which is converted to indole-3-acetonitrile (IAN) by successive action of the cytochrome P450 enzymes CYP79B2/B3 and CYP71A13. Extracts from wild-type plants and camalexin biosynthetic mutants, treated with silver nitrate or inoculated with Phytophthora infestans, were comprehensively analyzed by ultra-performance liquid chromatography elect ...[more]

PMID: 19567706

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