Ontology highlight
ABSTRACT:
INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - positive - reverse phase, Liquid Chromatography MS - negative - hilic
SUBMITTER: Sylvia Karin Neef
PROVIDER: MTBLS2130 | MetaboLights | 2021-10-12
REPOSITORIES: MetaboLights
Neef Sylvia K SK Janssen Nicole N Winter Stefan S Wallisch Svenja K SK Hofmann Ute U Dahlke Marc H MH Schwab Matthias M Mürdter Thomas E TE Haag Mathias M
Metabolites 20201201 12
As metabolic rewiring is crucial for cancer cell proliferation, metabolic phenotyping of patient-derived organoids is desirable to identify drug-induced changes and trace metabolic vulnerabilities of tumor subtypes. We established a novel protocol for metabolomic and lipidomic profiling of colorectal cancer organoids by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) facing the challenge of capturing metabolic information from a minimal sample amount (<500 cells/in ...[more]