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Proteomic Characterisation of Human Osteoblast-Derived Decellularised Extracellular Matrix for Osteogenic Repair

ABSTRACT: This project investigated cell-derived extracellular matrix (ECM) preparation for matrix-guided bone tissue engineering. A detergent-based decellularisation protocol using NH4OH and Triton X-100 was optimised using fibroblast-derived ECM to improve DNA removal while preserving ECM-associated protein content. To provide a more bone-relevant molecular reference, the same decellularisation approach was also applied to HOB-derived ECM. Proteomic analysis of HOB-derived dECM identified ECM-associated proteins linked to osteogenic activity, including collagens, laminins, annexins, and calcium-binding proteins. This dataset is presented as a descriptive reference for osteoblast-derived ECM composition, rather than as a direct comparison with the fibroblast-derived ECM used in functional assays.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Osteoblast, Cell Culture

SUBMITTER: Agata Szubska

LAB HEAD: Lucy Di Silvio

PROVIDER: PXD077920 | Pride | 2026-05-26

REPOSITORIES: Pride

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			Action	DRS
	PR857_AS1_AllSamples_Human_GO_20250613.sf3	Other
	PR857_AS1_AllSamples_Human_GO_NoScaling_20250624.mzML	Mzml
	PR857_AS1_AllSamples_Human_GO_NoScaling_20250624.mzid	Mzid
	PR857_AS1_AllSamples_Human_GO_NoScaling_20250624.xlsx	Xlsx
	PR857_AS1_HOB_C.msf	Msf

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