Metabolomics

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Metabolomic changes in Mycobacterium avium subsp. paratuberculosis (MAP) challenged Holstein-Friesian cattle suggest the role of serum amino acids as indicators of immune system activation


ABSTRACT:

INTRODUCTION: Paratuberculosis, commonly known as Johne’s disease, is a chronic intestinal infection of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). Clinical signs, including reduced milk yields, weight loss and diarrhoea, are typically absent until 2 to 6 years post exposure.

OBJECTIVES: To identify metabolomic changes profiles of MAP challenged Holstein-Friesian cattle and correlate identified metabolites to haematological and immunological parameters.

METHODS: At approximately 6 weeks of age, calves (n = 9) were challenged with 3.8 x 10^9 cells of MAP (clinical isolate CIT003) on 2 consecutive days. Additional unchallenged calves (n = 9) formed the control group. The study used biobanked serum from cattle sampled periodically from 3- to 33-months post challenge. The assessment of sera using flow infusion electrospray high resolution mass spectrometry (FIE-HRMS) for high throughput, sensitive, non-targeted metabolite fingerprinting highlighted differences in metabolite levels between the two groups.

RESULTS: In total, 25 metabolites which were differentially accumulated in MAP challenged cattle were identified, including 20 which could be correlated with certain haematological parameters, particularly monocyte levels.

CONCLUSION: The targeted metabolites suggest shifts in amino acid metabolism that could reflect immune system activation linked to MAP and as well as differences in phosphocholine levels which could reflect activation of the Th1 (tending towards pro-inflammatory) immune response. If verified by future work, selected metabolites could be used as biomarkers to diagnose and manage MAP infected cattle.

INSTRUMENT(S): Flow injection analysis MS - positive, Flow injection analysis MS - negative

SUBMITTER: Luis Mur 

PROVIDER: MTBLS2963 | MetaboLights | 2022-02-23

REPOSITORIES: MetaboLights

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