Metabolomics

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Comparative label-free lipidomic analysis of Mycobacterium tuberculosis during dormancy and reactivation

ABSTRACT: Mycobacterium tuberculosis employs several strategies to combat and adapt to adverse conditions encountered inside the host. The non-replicative dormant state of the bacterium is linked to drug resistance and slower response to anti-tubercular therapy. It is known that alterations in lipid content allow dormant bacteria to acclimatize to cellular stress. Employing comparative lipidomic analysis we profiled the changes in lipid metabolism in M. tuberculosis using a modified Wayne's model of hypoxia-induced dormancy. Further we subjected the dormant bacteria to resuscitation, and analyzed their lipidomes until the lipid profile was similar to that of normoxially grown bacteria. An enhanced degradation of cell wall-associated and cytoplasmic lipids during dormancy, and their gradual restoration during reactivation, were clearly evident. This study throws light on distinct lipid metabolic patterns that M. tuberculosis undergoes to maintain its cellular energetics during dormancy and reactivation.

INSTRUMENT(S): SYNAPT G2 HDMS (Waters)

PROVIDER: MTBLS304 | MetaboLights | 2018-10-22

REPOSITORIES: MetaboLights

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Dataset's files

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Action DRS
20150918_BR1_01_Control.raw.zip Raw
20150918_BR1_01_Dormant.raw.zip Raw
20150918_BR1_02_Control.raw.zip Raw
20150918_BR1_02_Dormant.raw.zip Raw
20150918_BR1_03_Control.raw.zip Raw
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Comparative label-free lipidomic analysis of Mycobacterium tuberculosis during dormancy and reactivation.

Raghunandanan Sajith S   Jose Leny L   Gopinath Vipin V   Kumar Ramakrishnan Ajay RA  

Scientific reports 20190306 1

Mycobacterium tuberculosis employs several strategies to combat and adapt to adverse conditions encountered inside the host. The non-replicative dormant state of the bacterium is linked to drug resistance and slower response to anti-tubercular therapy. It is known that alterations in lipid content allow dormant bacteria to acclimatize to cellular stress. Employing comparative lipidomic analysis we profiled the changes in lipid metabolism in M. tuberculosis using a modified Wayne's model of hypox  ...[more]

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