Metabolomics

Dataset Information

30

Comparative label-free lipidomic analysis of Mycobacterium tuberculosis during dormancy and reactivation.


ABSTRACT: Mycobacterium tuberculosis employs several strategies to combat and adapt to a variety of adverse conditions encountered inside the host. The non-replicative dormant state of the bacterium is linked to drug resistance and slower response to anti-tubercular therapy. It is known that alterations in lipid content allow dormant bacteria to acclimatize to cellular stress. Employing comparative lipidomic analysis, we profiled the changes in lipid metabolism in a modified Wayne’s model of hypoxia-induced dormancy. Further we subjected the dormant bacteria to resuscitation and analyzed the lipidomes of re-aerated bacteria till the lipid profiles were similar to that of normoxially grown bacteria. An enhanced degradation of cell wall associated and cytoplasmic lipids during dormancy and their gradual restoration during reactivation were clearly evident. This study throws light on distinct lipid metabolic patterns that MTB undergoes to maintain its cellular energetics during dormancy and reactivation.

INSTRUMENT(S): SYNAPT G2 HDMS (Waters)

SUBMITTER: Sajith Raghunandanan  

PROVIDER: MTBLS304 | MetaboLights | 2018-10-22

REPOSITORIES: MetaboLights

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