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Health outcomes of fruit juice and fruity beverage: a comparative analysis of gut microbiota and metabolomics in rats (Lipidomics assay)

ABSTRACT:

BACKGROUND: Diet is foods and drinks regularly consumed, and its regulation is complicated. It is necessary to obtain a practical understanding of which foods and drinks contribute to health and well-being and which are insalubrious. Clear evidence shows that a high intake of fruity beverage (FB) is associated with a higher risk of metabolic syndromes; unlike FB, the health outcome of fruit juice (FJ) intake is still controversial. Here, we aim to reveal the health outcomes of diet intervention (FJ or FB) with system profiling via the interaction of gut microbiota and metabolomics.

RESULTS: Firstly, the glucose, sucrose, fructose and other bioactive metabolites of FJ and FB were analyzed, and FJ possessed higher sucrose and flavonoids, while FB showed higher glucose and fructose. Secondly, C0 was set as the control group on Day 0, and a 4-week diet invention was performed to control, FJ-intake and FB-intake groups with normal saline, FJ and FB, respectively. Then, disparate trends in the metabolic syndrome of FJ-intake and FB-intake groups were revealed through the diet-microbiome-metabolic pathway axis. FJ improved alpha diversity and decreased the F/B ratio of gut microbiota as well as prevented insulin resistance with the reduced food intake in the FJ-intake group. However, FB possessed unchanged microbial diversity and enhanced F/B ratio, causing insulin resistance with renal triglyceride accumulation, increased kidney mass index as well as steadily improved weight change in FB-intake group.

CONCLUSIONS: The distinct processing degrees could explain the different FB and FJ intake responses in rats. FB with excess free fructose belongs to ultra-processed foods, while FJ with higher flavonoids belongs to minimally processed foods. We genuinely believe that FJ with a lower processing degree, although naturally contains the similar total amount of 3 main free sugars as FB, could be a healthier drink choice.

Lipidomics assay is reported in the current study MTBLS4108.

Metabolomics assay is reported in MTBLS3943.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Kewen Wang

PROVIDER: MTBLS4108 | MetaboLights | 2022-08-24

REPOSITORIES: MetaboLights

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Health outcomes of fruit juice and fruity beverage: a comparative analysis of gut microbiota and metabolomics in rats (Lipidomics assay)

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