Dataset Information

Mass spectrometry data of metabolomics analysis of Nepenthes pitchers

ABSTRACT: Hybridisation plays a significant role in the evolution and diversification of plants. Hybridisation among Nepenthes species is extensive, either naturally or man-made. To investigate the effects of hybridisation on the chemical compositions, we carried out metabolomics study on pitcher tissue of Nepenthes ampullaria, Nepenthes rafflesiana and their hybrid, Nepenthes × hookeriana. Pitcher samples were harvested and extracted in methanol:chloroform:water via sonication-assisted extraction before analysed using LC-TOF-MS. MS data were analysed using XCMS online version 2.2.5. This is the first MS data report towards the profiling, identification and comprehensive comparison of metabolites present in Nepenthes species.

INSTRUMENT(S): micrOTOF-Q II ESI-Qq-TOF (Bruker)

SUBMITTER: HOE HAN GOH

PROVIDER: MTBLS484 | MetaboLights | 2018-07-31

REPOSITORIES: MetaboLights

ACCESS DATA

Publications

Mass spectrometry data of metabolomics analysis of Nepenthes pitchers.

Rosli Muhammad Aqil Fitri MAF Azizan Kamalrul Azlan KA Baharum Syarul Nataqain SN Goh Hoe-Han HH

Data in brief 20170729

Hybridisation plays a significant role in the evolution and diversification of plants. Hybridisation among Nepenthes species is extensive, either naturally or man-made. To investigate the effects of hybridisation on the chemical compositions, we carried out metabolomics study on pitcher tissue of <i>Nepenthes ampullaria, Nepenthes rafflesiana</i> and their hybrid, <i>Nepenthes × hookeriana</i>. Pitcher samples were harvested and extracted in methanol:chloroform:water via sonication-assisted extr ...[more]

PMID: 28795107

Similar Datasets

Project description:Chromatin accessibility is an important functional genomics phenotype that influences transcription factor binding and gene expression. Genome-scale technologies allow chromatin accessibility to be mapped with high-resolution, facilitating detailed analyses into the genetic architecture and evolution of chromatin structure within and between species. We performed Formaldehyde-Assisted Isolation of Regulatory Elements sequencing (FAIRE-Seq) to map chromatin accessibility in two parental haploid yeast species, Saccharomyces cerevisiae and Saccharomyces paradoxus and their diploid hybrid. We show that although broad-scale characteristics of the chromatin landscape are well conserved between these species, accessibility is significantly different for 947 regions upstream of genes that are enriched for GO terms such as intracellular transport and protein localization exhibit. We also develop new statistical methods to investigate the genetic architecture of variation in chromatin accessibility between species, and find that cis effects are more common and of greater magnitude than trans effects. Interestingly, we find that cis and trans effects at individual genes are often negatively correlated, suggesting widespread compensatory evolution to stabilize levels of chromatin accessibility. Finally, we demonstrate that the relationship between chromatin accessibility and gene expression levels is complex, and a significant proportion of differences in chromatin accessibility might be functionally benign. There are 20 samples in total. These consist of 10 FAIRE-seq samples, specifically 6 haploid samples, S. cerevisiae strain UWOPS05_217_3 replicates 1 and 2, S. cerevisiae strain DBVPG1373 replicates 1 and 2, and S. paradoxus strain CBS432 replicates 1 and 2. There are also 4 diploid hybrid samples, hybrid between S. cerevisiae strain UWOPS05_217_3 and S. paradoxus strain CBS432 replicates 1 and 2, and the hybrid between S. cerevisiae strain DBVPG1373 and S. paradoxus strain CBS432 replicates 1 and 2. There are also RNA-seq samples for each of these 10 samples.

Dataset Information

Mass spectrometry data of metabolomics analysis of Nepenthes pitchers

Publications

Mass spectrometry data of metabolomics analysis of Nepenthes pitchers.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure