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Metabolomics analysis was used to resolve metabolites affecting the protein oil content of soybean.

ABSTRACT: Soybean (Glycine max (L.)) originated in China more than 5000 years ago, and the country is currently the third largest producer of soybeans in the world (Hymowitz et al., 2015). Based on a wild soybean Chromosome Segment Substitution Line (CSSL) population were constructed by crossing with the cultivar Suinong14 and ZYD00006 (the donor parent, a wild soybean variety) (Xin et al. 2016). A high-oil, low-protein (LPHO) and a high-protein, low-oil (HPLO) lines were screened out. In this study, a global interstitial microorganisms map were constructed, including Rhizosphere microorganisms, Phyllosphere microorganisms, Stem-interstitial microorganisms and interstitial microorganisms at the seeds developmental stage of early maturity stage (EM), mid-seed maturity stage (MM), late maturing stage (LM) and dry seed stage (DS). Whether the interstitial microorganisms may affect the growth of soybeans and the protein and oil content, as well as whether metabolites related to protein and oil content affect the surface and interior of soybeans in terms of beneficial microbial enrichment. To address these questions, we performed transcriptome sequencing, metabolomics, and microbial diversity analyses and examined significantly different microbial enrichment in LPHO/HPLO lines to screen out beneficial microbes associated with influencing soybean growth and affecting the protein and oil content. This finding will provide new ideas for further research on microorganisms affecting the protein and oil content of soybean seeds.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS9424 | MetaboLights | 2024-11-13

REPOSITORIES: MetaboLights

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	NEG_Fk29QZM_HPLODS_1.raw	Raw
	NEG_Fk29QZM_HPLODS_2.raw	Raw
	NEG_Fk29QZM_HPLODS_3.raw	Raw
	NEG_Fk29QZM_HPLODS_4.raw	Raw
	NEG_Fk29QZM_HPLODS_5.raw	Raw

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