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Omics score: 1024

Rice germination timecourse: Air-N2 gas switch

ABSTRACT: Rice seedling germinating in nitrogen gas (anaerobic) was transferred to air (aerobic) 24 hours after imbibition, and the changes in metabolite were tracked after 1, 3, 6, 24 hours of switch to aerobic condition.

OTHER RELATED OMICS DATASETS IN: E-MEXP-2267

INSTRUMENT(S): 1D-GC-EI-Q-MS

SUBMITTER: Adam J. Carroll

PROVIDER: MEX9 | MetabolomeExpress | 2009-07-01

REPOSITORIES: MetabolomeExpress

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Defining core metabolic and transcriptomic responses to oxygen availability in rice embryos and young seedlings.

Narsai Reena R Howell Katharine A KA Carroll Adam A Ivanova Aneta A Millar A Harvey AH Whelan James J

Plant physiology 20090701 1

Analysis reveals that there is limited overlap in the sets of transcripts that show significant changes in abundance during anaerobiosis in different plant species. This may be due to the fact that a combination of primary effects, changes due to the presence or absence of oxygen, and secondary effects, responses to primary changes or tissue and developmental responses, are measured together and not differentiated from each other. In order to dissect out these responses, the effect of the presen ...[more]

PMID: 19571305

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Project description:Transcript abundance profiles were examined over the first 24 hours of germination in rice grown under anaerobic conditions. Transcript abundance profiles were also examined for rice grown under aerobic conditions for 24 h and then switched to anaerobic conditions and vice versa.

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Microarray analysis of rice plants fumigated with ozone

Project description:In this study, integrated transcriptomics, proteomics and metabolomics approaches were applied to investigate the molecular responses of O3 in the leaves of two-weeks old rice (cv. Nipponbare) seedlings exposed to 0.2 ppm O3 for a period of 24 h. Based on the morphological alteration of O3-exposed rice leaves, transcript profiling of rice genes was performed in leaves exposed for 1, 12 and 24 h using rice DNA microarray chip, proteomics and metabolomics. This systematic survey showed that O3 triggers a chain reaction of altered gene, protein and metabolite expressions involved in multiple cellular processes in rice. Also investigated were the molecular responses in the leaves of two-weeks old rice (cv. Nipponbare) seedlings under continuous light and pure air (as a positive control for ozone exposure experiments) for a period of 24 h. Transcript profiling of rice genes was performed in leaves exposed for 1, 12 and 24 h using rice DNA microarray chip, proteomics and metabolomics. This systematic survey showed that continuous light and growth for 24 h also triggers a chain reaction of altered gene expressions involved in multiple cellular processes in rice, but different from those against ozone, in general. Keywords: Ozone fumigation response

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